巨大芽孢杆菌对江蓠植株琼脂直接脱硫工艺的建立及优化  

Establishment and Optimization of Technique for Direct Desulfation from Gracilaria Plant's Agar by Priestia megaterium

作　　者：宋阳 刘振 毛相朝[1] SONG Yang;LIU Zhen;MAO Xiangzhao(College of Food Science and Engineering,Ocean University of China,Qingdao 266403,Shandong,China)

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266403

出　　处：《食品研究与开发》2024年第2期177-184,共8页Food Research and Development

基　　金：国家重点研发计划项目(2021YFE0107500)。

摘　　要：为建立绿色便捷的琼脂脱硫工艺,以江蓠植株作为唯一硫源培养巨大芽孢杆菌进行琼脂脱硫。以脱硫菌的生长情况为评价指标,构建巨大芽孢杆菌对江蓠植株琼脂直接脱硫工艺,并优化江蓠植株脱硫工艺的灭菌方法、江蓠添加量和培养基各组分浓度。结果表明:江蓠植株琼脂在经过优化的脱硫工艺后,琼脂脱硫率达到37.985%,琼脂的凝胶强度由低于检测限提升至(200.263±7.121)g/cm^(2),发酵后的琼脂品质有明显的提升。To establish a green and efficient agar desulfation process,Priestia megaterium was cultured with Gracilaria plant as the sole sulfur source for agar desulfation.The growth of desulfating bacteria was taken as the evaluation index.A direct agar desulfation process using the Gracilaria plant was constructed,and the sterilization method,Gracilaria addition amount,and concentrations of various components in the culture medium for the desulfation process using the Gracilaria plant were optimized.The results showed that after the optimization of desulfation process,the desulfurization rate of agar from the Gracilaria plant reached 37.985%.The gel strength of the agar was increased from below the detection limit to(200.263±7.121)g/cm^(2),and the quality of the fermented agar was significantly improved.

关 键 词：江蓠 琼脂 微生物 脱硫 凝胶强度 

分 类 号：TS201.3[轻工技术与工程—食品科学]