嗜热链球菌m6A甲基化差异研究  

Studies on the Difference about m6A Methylation of Streptococcus thermophilus

作　　者：王丹[1] 孙志宏[1] 赵洁[1] Wang Dan;Sun Zhihong;Zhao Jie(Key Laboratory of Dairy Biotechnology and Engineering,Ministry of Education,Key Laboratory of Dairy Products Processing,Ministry of Agriculture and Rural Affairs,Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering,Cooperative Innovation Center of Lactic Acid Bacteria and Fermented Dairy Products,Inner Mongolia Agricultural University,Hohhot 010018)

机构地区：[1]内蒙古农业大学、乳品生物技术与工程教育部重点实验室、农业农村部乳制品加工重点实验室、内蒙古自治区乳品生物技术与工程重点实验室、乳酸菌与发酵乳制品省部共建协同创新中心,呼和浩特010018

出　　处：《中国食品学报》2023年第12期1-11,共11页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31901670);内蒙古自治区科技计划项目(2020GG0101);内蒙古自治区自然科学基金项目(2019BS03003)。

摘　　要：嗜热链球菌是乳制品工业生产中最为重要的发酵剂之一,具有良好的发酵特性。DNA甲基化是DNA化学修饰的一种形式,可在不改变DNA序列的前提下影响基因表达。本文选取2株产酸表型具有较大差异的嗜热链球菌为研究对象,通过甲基化组学探究m6A甲基化与遗传表型的关系。利用PacBio SMRT测序平台对嗜热链球菌IMAU80278与嗜热链球菌IMAU20416进行基因组测序及m6A甲基化测定,比较2株嗜热链球菌功能基因及m6A甲基化差异。结果表明,嗜热链球菌IMAU80278与嗜热链球菌IMAU20416基因组相似,且功能基因均以碳水化合物代谢及氨基酸代谢为主。嗜热链球菌IMAU80278与嗜热链球菌IMAU20416限制修饰系统,m6A甲基化位点和m6A基序均存在显著差异,特别是在碳水化合物代谢及氨基酸代谢相关功能基因的m6A甲基化存在明显差异。推测m6A甲基化的差异可能导致嗜热链球菌IMAU80278与嗜热链球菌IMAU20416产酸表型的不同。此外,从表观遗传学角度探究嗜热链球菌DNA甲基化对发酵特性的影响,为优良发酵剂的筛选及利用奠定理论基础。Streptococcus thermophilus is one of the most important starters in the dairy industry,with good fermentation characteristics.DNA methylation is a form of chemical modification of DNA that can affect gene expression without altering the DNA sequence.In this paper,two strains of Streptococcus thermophilus with large differences in acid-producing phenotype were selected as the research objects,and the relationship between m6A methylation and genetic phenotype was explored through methylome.The PacBio SMRT sequencing platform was used to sequence the genomes of Streptococcus thermophilus IMAU80278 and Streptococcus thermophilus IMAU20416 and measure the m6A methylation to compare the functional genes and m6A methylation differences between the two strains of Streptococcus thermophilus.The results showed that the genomes of Streptococcus thermophilus IMAU80278 and Streptococcus thermophilus IMAU20416 were similar,and the functional genes were mainly carbohydrate metabolism and amino acid metabolism.Streptococcus thermophilus IMAU80278 and Streptococcus thermophilus IMAU20416 showed significant differences in restriction modification systems,m6A methylation sites and m6A motifs,especially in the m6A methylation of genes related to carbohydrate metabolism and amino acid metabolism.It is speculated that the difference in m6A methylation may lead to the different acid-producing phenotypes between Streptococcus thermophilus IMAU80278 and Streptococcus thermophilus IMAU20416.In addition,this study explored the effect of Streptococcus thermophilus DNA methylation on fermentation characteristics from the perspective of epigenetics,and laid a theoretical foundation for the selection and utilization of excellent starter.

关 键 词：嗜热链球菌 m6A甲基化 限制修饰系统 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]