传统腌渍蔬菜中高产AI-2信号分子乳酸菌的筛选及其益生特性  

Screening of Lactic Acid Bacteria in Chinese Traditional Pickled Vegetableswith High Production of AI-2 Signal Molecule and Its Probiotic Properties

作　　者：吕欣然 刘雪晴 顾振辰 吕孟敏 励建荣 檀茜倩 崔方超 白凤翎 俞张富 沈荣虎 LüXinran;Liu Xueqing;Gu Zhenchen;LüMengmin;Li Jianrong;Tan Xiqian;Cui Fangchao;Bai Fengling;Yu Zhanfu;Shen Ronghu(National&Local Joint Engineering Research Center of Storage,Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products,Food Safety Key Lab of Liaoning Province,College of Food Science and Engineering of Bohai University,Jinzhou 121013,Liaoning;Hangzhou Xiaoshan Agriculture Development Co.,Ltd.,Hangzhou 311215)

机构地区：[1]渤海大学食品科学与工程学院、生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心、辽宁省食品安全重点实验室,辽宁锦州121013 [2]杭州萧山农业发展有限公司,杭州311215

出　　处：《中国食品学报》2024年第1期83-91,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：辽宁省博士科研启动基金计划项目(2020-BS-236);辽宁省食品安全重点实验室开放课题(LNKLFS202102)。

摘　　要：LuxS/AI-2作为种间交流群体感应系统常存在于乳酸菌中,具有调控其生物膜形成,耐酸、耐胆盐等益生特性。采用哈维氏弧菌BB170菌株报告法筛选产AI-2信号分子乳酸菌,利用PCR测序检测luxS基因的表达,通过耐酸、耐胆盐、抑菌性及细胞黏附性评价高产AI-2乳酸菌的益生特性。结果表明:从200株乳酸菌中筛选出10株产AI-2乳酸菌,其中植物乳杆菌SCT-2为高产AI-2信号菌株。验证菌株SCT-2中存在luxS群体感应调控基因。高产AI-2菌株SCT-2的耐酸和耐胆盐存活率分别为14%~92%和63%~89%,抑菌直径范围17.50~19.43 mm,黏附细胞数为102 CFU/细胞。与低产AI-2乳酸菌菌株比较,生物膜产量、耐酸、耐胆盐、抑菌率及黏附率分别提高111.4%,8%,15%,26.7%,197%。结论:luxS基因是AI-2信号合成的关键基因,LuxS/AI-2群体感应提高了乳酸菌的生物膜形成能力、耐酸性、耐胆盐性、抑菌能力及细胞黏附力等益生性,为开发良好益生特性的乳酸菌生物制剂提供了理论参考。LuxS/AI-2,as an interspecific communication quorum sensing(QS)system,is commonly found in lactic acid bacteria(LAB),which can regulate the probiotic properties of LAB,including biofilm formation,acid tolerance,bile salt tolerance,cells adhesion.In this study,LAB with production of AI-2 signal molecule was screened using strain Vibrio harveyi BB170 reporting method.The expression of luxS gene was detected by PCR sequencing.Moreover,the probiotic properties of high-yield AI-2 LAB were evaluated by acid resistance,bile salt resistance,antibacterial and cells adhesion.The results showed that ten strains of LAB producing AI-2 were screened from two hundred strains LAB.Among them,Lactobacillus plantarum SCT-2 had strong ability to produce AI-2 signal molecule.The luxS gene was further verified exist in strain SCT-2.The survival rates of acid and bile salt resistant in strain SCT-2 were 14%-92%and 63%-89%,respectively.The antibacterial diameter of strain SCT-2 ranged from 17.50 mm to 19.43 mm.The number of adhesion cells of strain SCT-2 was 102 CFU/cell.Compared to LAB with low production AI-2,the biofilm formation,acid tolerance,bile salt tolerance,inhibitory rate and cell adhesion of strain SCT-2 increased by 111.4%,8%,15%,26.7%and 197%,respectively.These results indicated that luxS gene was the key gene of AI-2 signal synthesis.LuxS/AI-2 quorum sensing system improves the biofilm forming ability,acid tolerance,bile salt tolerance antibacterial ability and cells adhesion of LAB.It will provide a theoretical basis for the development of LAB biological agent with good probiotic properties.

关 键 词：乳酸菌 群体感应 AI-2信号分子 益生特性 

分 类 号：TS255.5[轻工技术与工程—农产品加工及贮藏工程]