酶法辅助提取人工培育蝉花多糖工艺优化及其动力学、热力学、抗氧化研究  

Process Optimization of Enzyme-Assisted Extraction of Polysaccharides from Artificially-Cultivated Cordyceps cicadae and Its Kinetic,Thermodynamic and Antioxidant Activities Analysis

作　　者：谢娜 程俊文[2] 徐娟[1] 吴学谦[1] 李春如 王玉芹 熊科辉 贺亮[2] XIE Na;CHENG Junwen;XU Juan;WU Xueqian;LI Chunru;WANG Yuqin;XIONG Kehui;HE Liang(Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine,College of Food and Health,Zhejiang A&F University,Hangzhou 311300,China;Zhejiang Provincial Key Laboratory of Biological and Chemical Utilization of Forestry Resources,Zhejiang Academy of Forestry,Hanghzou 310023,China;Bioasia Life Institute,Zhejiang Bioasia Pharmaceutical Co.,Ltd.,Jiaxing 314200,China;Zhejiang Wuyangtang Pharmaceutical Co.,Ltd.,Lishui 323000,China)

机构地区：[1]浙江省特色中药资源保护与创新利用重点实验室,浙江农林大学食品与健康学院,浙江杭州311300 [2]浙江省森林资源生物与化学利用重点实验室,浙江省林业科学研究院,浙江杭州310023 [3]泛亚生命科学研究院,浙江泛亚生物医药股份有限公司,浙江嘉兴314200 [4]浙江五养堂药业有限公司,浙江丽水323000

出　　处：《食品工业科技》2024年第4期151-160,共10页Science and Technology of Food Industry

基　　金：浙江省科技计划项目(2021F1065-8)。

摘　　要：目的:优化酶法辅助提取人工培育蝉花多糖的工艺,考察料液比、酶添加量、酶解温度、提取时间等单因素对多糖得率的影响。方法:借助Box-Behnken设计的四因素三水平法建立了该多糖提取工艺的理论模型,并研究多糖对三种自由基的清除效果,根据Fick第二定律建立了人工培育蝉花提取的动力学模型,并根据速率常数、相对萃余率、活化能等参数,研究其动力学和热力学性质。结果:经响应面分析得到该多糖最佳提取工艺为:料液比1:30 g/mL、酶添加量1.6%、酶解温度67℃、提取时间90 min,在此条件下蝉花多糖的得率可达7.91%,与理论值接近。在该工艺下的人工蝉花粗多糖的DPPH自由基和羟自由基的IC_(50)值分别为0.54和0.60 mg/mL,ORAC值是45.62 Troloxμmol/g,具有良好的体外抗氧化活性。结论:利用酶法辅助提取人工培育蝉花多糖,为该类已商业化的蝉花资源的多糖组分开发提供了理论基础。Objective:To optimize the enzyme-assisted extraction process of polysaccharides from artificially-cultivated Cordyceps cicadae was conducted in this study.Four different parameters were considered to evaluate the yield of polysaccharides including liquid to solid ratio,enzyme dosage,enzymatic temperature and extraction time.Methods:A four-factor-three-level experimental design was employed to establish a mathematical model by Box-Behnken method,and the scavenging capacity of polysaccharides against three radicals was examined.Afterward,Fick's second law was used to build the kinetic model for the extraction of polysaccharide from artificially-cultivated Cordyceps cicadae.The parameters including rate constants,relative extraction rate,and activation energy were employed to analyze the kinetic and thermodynamic features.Results:Based on the response surface analysis,the optimal extraction process was presented to be as following:Liquid to solid ratio 1:30 g/mL,enzyme dosage 1.6%,enzymatic digestion temperature 67℃and extraction time 90 min.The polysaccharide yield under the above condition was 7.91%,which was close to the predicted value.Moreover,the results of antioxidant capacities indicated that the obtained crude polysaccharides under optimal conditions showed strong DPPH radical scavenging and hydroxyl radical scavenging with IC_(50) values for 0.60 and 0.54 mg/mL,respectively,and its ORAC value was 45.62 Troloxμmol/g,suggesting potent antioxidant activity in vitro.Conclusion:The study of enzyme-assisted extraction of artificially-cultivated Cordyceps cicadae flower polysaccharides provide theoretical support for the production of polysaccharide fractions from this kind of commercialized Cordyceps cicadae resources.

关 键 词：人工培育蝉花多糖 提取 响应面法 动力学 热力学 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]