基于整体法的食品中大肠菌群测量不确定度评定  

Evaluation of Uncertainty in Measurement of Coliform Bacteria in Food Based on Holistic Method

作　　者：陈晶 CHEN Jing(Mingchengtengde Testing Service(Fuzhou)Co.,Ltd.,Fuzhou 350008,China)

机构地区：[1]名成腾德检测服务(福州)有限公司,福建福州350008

出　　处：《现代食品》2023年第22期197-199,共3页Modern Food

摘　　要：本文采用平板计数法,以人工污染大肠菌群羊肉样品为例,测量了不确定度,并评定了数据的离散型。结果表明,试验不确定度来源为样品制备的均质性、标准菌株的接种量波动、培养基制备与pH调节的精确度、恒温培养条件的一致性、菌落数计数的人为误差,以及仪器的校准误差。This article uses the plate counting method to measure the uncertainty and evaluate the discreteness of the data using artificially contaminated sheep meat samples of coliform bacteria.The results showed that the sources of experimental uncertainty were the homogeneity of sample preparation,fluctuations in the inoculation amount of standard strains,accuracy of culture medium preparation and pH adjustment,consistency of constant temperature culture conditions,human error in colony count and calibration error of instruments.

关 键 词：整体法 大肠菌群 食品微生物检测 

分 类 号：TS207.4[轻工技术与工程—食品科学]