大米肽螯合物的体外抗氧化作用研究  

Study on Antioxidant Effects of Rice Peptide Chelate in Vitro

作　　者：刘建 段升霞 陈浏安 张东立 张大虎 赵贵红[1] LIU Jian;DUAN Shengxia;CHEN Liuan;ZHANG Dongli;ZHANG Dahu;ZHAO Guihong(College of Agriculture and Bioengineering,Heze University,Heze 274000;College of Chemistry and Chemical Engineering,Heze University,Heze 274000;Shandong Bigtree Dafu Co.,Ltd.Majoring Foods for Special Dietary Uses,Heze 274000)

机构地区：[1]菏泽学院农业与生物工程学院,菏泽274000 [2]菏泽学院化工学院,菏泽274000 [3]山东大树达孚特膳食品有限公司,菏泽274000

出　　处：《食品工业》2024年第1期26-31,共6页The Food Industry

基　　金：菏泽市科技创新突破计划项目(2019CXTP08);山东省科技型中小企业创新能力提升工程项目(2021TSGC1254);2021年度山东省重点研发计划(科技示范工程)项目(2021SFC1205)。

摘　　要：以大米肽、氯化钙、硫酸锌和硫酸亚铁为原料,经螯合工艺分别制备大米肽钙、锌、铁螯合物。对大米肽的分子量分布和氨基酸组成进行了检测,然后采用ABTS、DPPH自由基清除试验和还原力试验考察对比大米肽及其螯合物的体外抗氧化功能。结果表明,大米肽分子量分布范围主要在200~2000 Da之间,分子量小于2000 Da的大米肽占总肽量的比例约为82.02%,大米肽氨基酸含量最高的为谷氨酸。大米肽及其螯合物均表现出良好的体外抗氧化性,且均随着浓度的增加而上升,在一定浓度范围内,大米肽钙锌铁螯合物清除DPPH自由基、ABTS自由基能力及还原能力均比大米肽有所提高。红外光谱检测结果表明,大米肽与钙、锌、铁的螯合后,大米肽中基团与金属离子成功结合,形成了新的不同结构。Use rice peptide,calcium chloride,zinc sulfate,and ferrous sulfate as raw materials to prepare rice peptide calcium,zinc,and iron chelates through chelation processes.The molecular weight distribution and amino acid composition of rice peptides were conducted,and the antioxidant function in vitro of rice peptides and their chelates was compared using ABTS,DPPH radical scavenging experiments,and reducing power experiments.The results showed that the molecular weight distribution range of rice peptides was mainly between 200 Da and 2000 Da,and the proportion of rice peptides with molecular weight less than 2000 Da to the total peptide weight was about 82.02%.The highest amino acid content of rice peptides was glutamic acid.Both rice peptides and their chelates exhibited good antioxidant properties in vitro,and increased with their concentration.Within a certain concentration range,the ability of rice peptide calcium zinc iron chelates to scavenge DPPH free radicals,ABTS free radicals,and reducing power was improved compared to rice peptides.The infrared spectrum detection results indicated that after the chelation of rice peptide with calcium,zinc,and iron,the functional groups in rice peptide successfully were bound with metal ions,forming new and different structures.

关 键 词：大米肽 螯合物 抗氧剂 红外光谱 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]