天然低共熔溶剂促进纤维素酶水解橄榄苦苷的研究  

Study on the promoting effect of natural deep eutectic solvent on cellulase hydrolysis of oleuropein

作　　者：王明明 陈根振 胡锦霞 曾韦丹 王晗 裴栋[2,3] 曲清莉 邸多隆 WANG Ming-ming;CHEN Gen-zhen;HU Jin-xia;ZENG Wei-dan;WANG Han;PEI Dong;QU Qing-li;DI Duo-long(College of Pharmacy,Gansu University of Chinese Medicine;CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province,Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou 730000,China;Qingdao Center of Resource Chemistry and New Materials,Qingdao 266000,China;Yunnan Olive Health Industry Innovation Research and Development Co.,Ltd.,Lijiang 674100,China)

机构地区：[1]甘肃中医药大学药学院 [2]中国科学院兰州化学物理研究所中国科学院西北特色植物资源化学重点实验室和甘肃省天然药物重点实验室,兰州730000 [3]青岛市资源化学与新材料研究中心,青岛266000 [4]云南油橄榄大健康产业创新研究发展有限公司,丽江674100

出　　处：《天然产物研究与开发》2024年第2期314-321,335,共9页Natural Product Research and Development

基　　金：甘肃省科技重大专项(22ZD6FA021);兰州市科技计划(2022-2-2);甘肃省重点研发计划(22YF7NA027);云南省重点研发计划(202203AD150012)。

摘　　要：橄榄苦苷酶水解产物具有良好的生物活性,为了促进纤维素酶水解橄榄苦苷,本研究通过天然低共熔溶剂(natural deep eutectic solvent,NADES)对纤维素酶稳定性的促进作用,形成NADES-纤维素酶溶剂系统促进橄榄苦苷的酶水解。以橄榄苦苷酶水解率为指标,考察温度、pH和DES种类及浓度对纤维素酶水解橄榄苦苷的影响。结果表明:在50℃,pH=5时水解3 h,以10%(V/V)DES-5(甜菜碱∶1,4-丁二醇=1∶2,n/n)为溶剂系统的橄榄苦苷酶水解率是缓冲液的1.7倍;证明以氯化胆碱或甜菜碱为氢键受体(hydrogen bond acceptor,HBA),多元醇为氢键供体(hydrogen bond donor,HBD)的NADES对增强纤维素酶稳定性、促进橄榄苦苷酶水解具有良好的效果,为高效制备橄榄苦苷酶水解产物提供了科学依据。Oleuropein hydrolyzed products exhibit potent biological activities.To enhance oleuropein hydrolysis by cellulase,a natural deep eutectic solvent(NADES)was utilized to stabilize the cellulase enzyme.The DES-cellulase solvent system effectively promoted enzymatic hydrolysis of oleuropein.Factors such as temperature,pH,DES type and concentration were studied to determine their impact on the hydrolysis rate.Results showed that at 50℃and pH=5,the hydrolysis rate of oleuropein was 1.7 times higher than that of buffer solution when using 10%(V/V)DES-5(betaine:1,4-butanediol=1∶2,n/n)as solvent for 3 h.This study confirms that NADESs with choline chloride or betaine as hydrogen bond acceptor(HBA)and polyols as hydrogen bond donor(HBD)effectively enhance cellulase stability and promote oleuropein hydrolysis.These findings provide a scientific basis for the efficiently producing oleuropein enzyme hydrolyzed products.

关 键 词：低共熔溶剂 橄榄苦苷 纤维素酶 酶水解 

分 类 号：R914[医药卫生—药物化学]