微波辅助顶空固相微萃取GC-MS法分析柠檬草中挥发性成分  

Microwave Assisted Headspace Solid-phase Microextraction GC-MS Analysis of Volatile Components in Lemon Grass

作　　者：杨玉贤 张琼 杨绍坤 高云涛[2] 和顺琴 Yang Yuxian;Zhang Qiong;Yang Shaokun;Gao Yuntao;He Shunqin(Yunnan Medical and Health Vocational College,Kunming 650033,China;Yunnan University for Nationalities,Kunming 650500,China)

机构地区：[1]云南医药健康职业学院,云南昆明650033 [2]云南民族大学,云南昆明650500

出　　处：《云南化工》2024年第2期93-99,共7页Yunnan Chemical Technology

摘　　要：设计制作了一种微型底聚焦微波反应装置,用于辅助顶空固相微萃取,解决了无溶剂顶空提取的问题,建立了柠檬草样品中挥发性成分的微波辅助顶空固相微萃取方法。使用GC-MS法对柠檬草叶片和根茎萃取物中挥发性成分进行分析鉴定,分别得到37和46种化合物,结合峰面积归一法确定各组分的含量,其挥发性成分别占萃取物挥发总量的89.93%和77.29%。其中叶片和根茎中含量较高的挥发性成分都是橙花醛和香叶醛,叶片中的主要挥发性成分橙花醛(29.27%)和香叶醛(22.14%)的相对含量要明显优于根茎中橙花醛(8.61%)和香叶醛(7.76%)。研究为样品特别是固体样品挥发性成分的直接快速分析提供了一种稳定高效、简单快速的新方法,具有广泛的应用前景。A micro microwave reaction device was designed and manufactured to assist headspace solid-phase microextraction,solving the problem of solvent-free headspace extraction.A microwave assisted headspace solid-phase microextraction method for volatile components in lemon grass samples was established.Using GC-MS method to analyze and identify the volatile components in the extracts of lemon grass leaves and roots,37 and 46 types were identified,and the content of each component was determined by peak area normalization method.37 volatile compounds in lemon grass leaves and 46 volatile compounds in roots and stems account for 89.93%and 77.29%of the total volatile compounds in the extract,respectively.Among them,the volatile components with higher content in the leaves and rhizomes are both nerol and vanillin.The relative content of the main volatile components in the leaves,nerol(29.27%)and vanillin(22.14%),is significantly higher than that in the rhizomes,nerol(8.61%)and vanillin(7.76%).This study provides a stable,efficient,simple,and fast new method for the direct and rapid analysis of volatile components in samples,especially in solid samples,with broad application prospects.

关 键 词：微型底聚焦微波反应器 顶空固相微萃取 气相色谱-质谱联用 柠檬草 挥发性成分 

分 类 号：O657[理学—分析化学] TQ654[理学—化学]