Cav3.2 channel regulates cerebral ischemia/reperfusion injury:a promising target for intervention  

作　　者：Feibiao Dai Chengyun Hu Xue Li Zhetao Zhang Hongtao Wang Wanjun Zhou Jiawu Wang Qingtian Geng Yongfei Dong Chaoliang Tang 

机构地区：[1]Graduate School,Wannan Medical College,Wuhu,Anhui Province,China [2]Department of Anesthesiology,The First Affiliated Hospital of USTC,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei,Anhui Province,China [3]Core Facility Center for Medical Sciences,The First Affiliated Hospital of USTC(Anhui Provincial Hospital),Hefei,Anhui Province,China [4]Department of Pharmacy,The First Affiliated Hospital of USTC,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei,Anhui Province,China [5]Department of Neurosurgery,The First Affiliated Hospital of USTC,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei,Anhui Province,China

出　　处：《Neural Regeneration Research》2024年第11期2480-2487,共8页中国神经再生研究（英文版）

基　　金：supported by the Natural Science Foundation of Anhui Province of China,No.2208085Y32;Scientific Research Plan Project of Anhui Province of China,No.2022AH020076;the Chen Xiao-Ping Foundation for the Development of Science and Technology of Hubei Province,No.CXPJJH12000005-07-115(all to CT).

摘　　要：Calcium influx into neurons triggers neuronal death during cerebral ischemia/reperfusion injury.Various calcium channels are involved in cerebral ischemia/reperfusion injury.Cav3.2 channel is a main subtype of T-type calcium channels.T-type calcium channel blockers,such as pimozide and mibefradil,have been shown to prevent cerebral ischemia/reperfusion injury-induced brain injury.However,the role of Cav3.2 channels in cerebral ischemia/reperfusion injury remains unclear.Here,in vitro and in vivo models of cerebral ischemia/reperfusion injury were established using middle cerebral artery occlusion in mice and high glucose hypoxia/reoxygenation exposure in primary hippocampal neurons.The results showed that Cav3.2 expression was significantly upregulated in injured hippocampal tissue and primary hippocampal neurons.We further established a Cav3.2 gene-knockout mouse model of cerebral ischemia/reperfusion injury.Cav3.2 knockout markedly reduced infarct volume and brain water content,and alleviated neurological dysfunction after cerebral ischemia/reperfusion injury.Additionally,Cav3.2 knockout attenuated cerebral ischemia/reperfusion injury-induced oxidative stress,inflammatory response,and neuronal apoptosis.In the hippocampus of Cav3.2-knockout mice,calcineurin overexpression offset the beneficial effect of Cav3.2 knockout after cerebral ischemia/reperfusion injury.These findings suggest that the neuroprotective function of Cav3.2 knockout is mediated by calcineurin/nuclear factor of activated T cells 3 signaling.Findings from this study suggest that Cav3.2 could be a promising target for treatment of cerebral ischemia/reperfusion injury.

关 键 词：CALCINEURIN Cav3.2 channel cerebral ischemia/reperfusion hippocampus HYPOXIA/REOXYGENATION inflammatory response nuclear factor of activated T cells 3 oxidative stress primary hippocampal neurons stroke 

分 类 号：R743[医药卫生—神经病学与精神病学]