高效液相色谱法测定食品中4-己基间苯二酚残留量的方法验证  

Validation of Method for Determination of 4-Hexylresorcinol Residues in Food by High Performance Liquid Chromatography

作　　者：王韵 李巧莲 林洋 郁蕉竹 鞠晓莹 党辉 WANG Yun;LI Qiaolian;LIN Yang;YU Jiaozhu;JU Xiaoying;DANG Hui(Shenyang Food and Drug Inspection Institute,Shenyang 110122,China;Liaoning Open University,Shenyang 110034,China)

机构地区：[1]沈阳市食品药品检验所,辽宁沈阳110122 [2]辽宁开放大学,辽宁沈阳110034

出　　处：《食品安全导刊》2024年第1期93-96,共4页China Food Safety Magazine

摘　　要：目的:验证沈阳市食品药品检验所实验室是否有开展《食品安全国家标准食品中4-己基间苯二酚残留量的测定》(GB 5009.280—2020)项目的检验能力。方法:采用高效液相色谱进行分离,荧光检测器进行检测分析,根据保留时间进行定性分析,外标法进行准确定量,同时验证方法标准曲线线性范围、检出限、定量限、精密度和回收率。结果:当4-己基间苯二酚浓度在0.02~2.00 mg·L^(-1)时,呈现出的线性关系良好,方法检出限、定量限、精密度和回收率均能满足国家标准方法要求。结论:沈阳市食品药品检验所实验室有能力进行食品中4-己基间苯二酚残留量的测定。Objective:To verify whether the laboratory of Shenyang Institute for Food and Drug Control has the ability to carry out the GB 5009.280—2020 project.Method:High performance liquid chromatography was used for separation,fluorescence detector was used for detection and analysis,qualitative analysis was carried out according to the retention time,and external standard method was used for accurate quantification.At the same time,the linear range,detection limit,quantitative limit,precision and recovery rate of the standard curve of the method were verified.Result:When the concentration of 4-hexylresorcinol is in the range of 0.02~2.00 mg·L^(-1),the linear relationship is good.The detection limit,quantitative limit,precision and recovery rate of the method can meet the requirements of national standard method.Conclusion:The laboratory of Shenyang Food and Drug Inspection Institute has the ability to determine the residual amount of 4-hexylresorcinol in food.

关 键 词：高效液相色谱 食品 4-己基间苯二酚 方法验证 

分 类 号：TS207.5[轻工技术与工程—食品科学] O657.72[轻工技术与工程—食品科学与工程]