低浓度微酸性电解水对纯培养及海虾中副溶血性弧菌与腐败希瓦氏菌的杀灭作用  

Bactericidal effect of low concentration slightly acidic electrolyzed water on Vibrio parahaemolyticus and Shewanella putrefaciens in pure culture and shrimp

作　　者：刘阳 王程可苡 杨宇 罗宗洪 常冠红 王新[1] 张春玲 LIU Yang;WANG Chengkeyi;YANG Yu;LUO Zonghong;CHANG Guanhong;WANG Xin;ZHANG Chunling(College of Food Science and Engineering,Northwest A&F University,Yangling 712100,China)

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100

出　　处：《食品与发酵工业》2024年第4期51-58,共8页Food and Fermentation Industries

基　　金：国家自然科学基金项目(31871894,U1703119)。

摘　　要：为探究低浓度微酸性电解水(low concentration slightly acidic electrolyzed water,LcSAEW)对纯培养及海虾中的副溶血性弧菌与腐败希瓦氏菌的杀灭作用,该研究通过平板计数、生长曲线、电导率、膜电位、胞内物质渗漏量、活性氧和显微镜观察分析了LcSAEW对两种细菌的抑菌效果、细胞膜完整性以及细胞形态的影响。结果表明,有效氯浓度为4、8 mg/L的LcSAEW处理纯培养副溶血性弧菌与腐败希瓦氏菌30 s时,菌落数均降低8 lg CFU/mL以上。用10 mg/L的LcSAEW处理接种两种目标菌的海虾10 min后,菌落数分别降低1.6、1.25 lg CFU/mL。LcSAEW处理后,2种细菌的代谢活性和胞内ATP浓度均降低,而电导率、胞外DNA、蛋白质和K^(+)含量均上升。此外,膜电位呈现超极化,胞内活性氧累积,细胞形态出现凹陷、皱缩现象。综上所述,LcSAEW可以破坏细胞结构、细胞膜通透性,导致细菌活性紊乱甚至凋亡。该研究可为LcSAEW在水产品中的应用提供理论依据。This work was aim to investigate the bactericidal effect of low concentration slightly acidic electrolyzed water(LcSAEW)against Vibrio parahaemolyticus and Shewanella putrefaciens in pure culture and shrimp.In this study,the effects of LcSAEW on the antibacterial effect,cell membrane integrity and cell morphology of both bacteria were analyzed by plate counts,growth curves,conductivity,membrane potential,leakage of intracellular material,reactive oxygen species(ROS)and microscopic observation.The results showed that when pure cultures of V.parahaemolyticus and S.putrefaciens were treated with LcSAEW at available chlorine concentrations of 4 or 8 mg/L for 30 s,the colony count decreased by more than 8 lgCFU/mL.Treatment of shrimp inoculated with two target bacteria with 10 mg/L LcSAEW for 10 min resulted in a reduction in colony counts of 1.6 and 1.25 lgCFU/mL,respectively.The metabolic activity and intracellular ATP concentration were observed decreased in both type of bacteria,whereas,the conductivity,extracellular DNA,protein and K^(+)contents increased by LcSAEW treatment.In addition,the membrane potential showed hyperpolarization,the intracellular ROS accumulated,and the cell morphology appeared depression and shrinkage.In summary,LcSAEW can destroy cell structure,cell membrane permeability,and therefore,result in disturbance of bacterial activity,and even apoptosis occurs.This study provides a theoretical basis for the application of LcSAEW in aquatic products.

关 键 词：低浓度微酸性电解水 副溶血性弧菌 腐败希瓦氏菌 杀灭效果 作用机制 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]