酵母强化大曲接种量优化及其生产应用  

Optimization of inoculation amount and its production application of yeast-fortified Daqu

作　　者：张军 孙腾 张翠云 董培方 朱立宁 马辉峰 ZHANG Jun;SUN Teng;ZHANG Cuiyun;DONG Peifang;ZHU Lining;MA Huifeng(Hebei Fenglaiyi Wine Industry Co.,Ltd.,Xingtai 055550,China)

机构地区：[1]河北凤来仪酒业有限公司,河北邢台055550

出　　处：《中国酿造》2024年第2期206-210,共5页China Brewing

摘　　要：为提高泥坑浓香型白酒中高温大曲综合生产性能,该研究从中高温大曲中分离筛选菌株,采用2,3,5-氯化三苯基四氮唑(TCC)初筛,发酵实验复筛,获得发酵性能良好的酵母菌,并以Z综合得分为响应值,通过单因素试验及响应面试验对筛选菌株接种量进行优化,并将其用于强化大曲生产。结果表明,共分离纯化出8株菌株,其中,3株产酒性能良好的菌株Y1、Y2、Y3,其最佳接种量分别为7.0×10^(8)CFU/mL、3.0×10^(8)CFU/mL、1.5×10^(8)CFU/mL,在此优化复配条件下,主成分Z综合得分为513,所生产的强化大曲发酵力、糖化力和液化力分别为3.46 g/(g·72 h)、994 mg/(g·h)、1.12 g/(g·h),比优化前提高了424%、6.7%和5.7%。In order to improve the comprehensive production performance of medium-high-temperature Daqu in strong-flavor(nongxiangxing)Baijiu,strains were separated and screened from medium-high-temperature Daqu,and 2,3,5-triphenyltetrazolium chloride(TCC)was used for preliminary screening,and the fermentation experiments were used for rescreening to obtain yeast with good fermentation performance.The Z comprehensive score was used as the response value.Through single factor and response surface test,the inoculation of screened strains was optimized,and it was used to strengthen Daqu production.The results showed that a total of 8 strains were isolated and purified,among which 3 strains of yeast Y1,Y2,and Y3 with good alcohol production performance were screened out.The inoculum of 3 strains of yeast was 7.0×10^(8) CFU/ml,3.0×10^(8) CFU/ml,1.5×10^(8) CFU/ml.Under the optimized combination conditions,the overall score of principal component Z was 513.The fermentation power,saccharification power and liquefaction power were 3.46 g/(g·72 h),994 mg/(g·h)and 1.12 g/(g·h),respectively,which were increased by 424%,6.7%and 5.7%compared with before optimization.

关 键 词：高温大曲 筛选酵母 配比 响应面优化 应用 

分 类 号：TS262.3[轻工技术与工程—发酵工程]