乳酸菌发酵对鸡骨泥钙释放及代谢物的影响  

Effects of Lactic Acid Bacteria Fermentation on Calcium Releas e and Metabolites in Chicken Bone Paste

作　　者：王明[1] 夏强 孙杨赢[1] 何俊 潘道东[1] 曹锦轩[1] 周昌瑜 WANG Ming;XIA Qiang;SUN Yangying;HE Jun;PAN Daodong;CAO Jinxuan;ZHOU Changyu(College of Food and Pharmaceutical Sciences,Ningbo Universit y,Key Laboratory of Animal Products Food Deep Processing Technology of Zhejiang Province,Ningbo 315211,Chin a)

机构地区：[1]宁波大学食品与药学学院,浙江省动物蛋白精深加工重点实验室,浙江宁波315211

出　　处：《食品工业科技》2024年第6期67-75,共9页Science and Technology of Food Industry

基　　金：国家自然科学基金(32101975);浙江省自然科学基金(LQ22C200017)。

摘　　要：本研究以鸡骨为原料,研究了嗜酸乳杆菌(LA)、罗伊氏乳杆菌(LR)和植物乳杆菌(LP)作为发酵剂对鸡骨泥中钙释放及代谢物的影响,通过监测菌株的生长状态、发酵液的pH、总酸变化、钙分布和钙磷比、代谢物种类及含量揭示乳酸菌的生长特性变化及其对钙释放的影响,并借助主成分分析、相关性分析以及KEGG代谢通路分析挖掘乳酸菌发酵骨泥的关键代谢通路。结果表明,LA、LR和LP均能较好的利用鸡骨泥进行生长繁殖,且在发酵30 h时活菌数达最大值,LA、LP和LR发酵组的总酸含量分别为5.60、3.76和3.75 g/L,LA发酵组的总酸含量显著高于其他处理组(P<0.05)。钙释放分析表明,LR组、LP组和LA组总钙含量分别从181.33 mg/kg(对照组)上升至1176.67、1310.00和1916.67 mg/kg;游离钙含量分别是对照组的40.60、50.19和74.62倍,且LA发酵组的游离钙含量显著高于LP和LR发酵组(P<0.05)。X射线衍射和红外光谱结果表明,发酵骨泥中羟基磷灰石主要以无定型形态存在,与对照组相比,LR组、LP组和LA组中羟基磷灰石的特征峰在2926和1050 cm^(-1)处的强度明显降低。LC-MS/MS结果显示,乳酸菌发酵后骨泥中共鉴定出37种代谢物,PCA和相关性分析代谢物的特征发现,乳酸、丙酮酸、蔗糖、丝氨酸、5'-CMP等是其关键代谢物。KEGG代谢物通路分析表明,嘧啶代谢、精氨酸和脯氨酸代谢、糖酵解、丙酮酸代谢、牛磺酸代谢和TCA循环是其主要代谢通路,这些代谢通路主要与乳酸菌的生长以及骨泥中钙释放密切相关。The effects of Lactobacillus acidophilus CICC6074(LA),Limosilactobacillus reuteri WQ-Y1(LR)and Lactiplantibacillus plantarum A3(LP)on calcium release and metabolite composition during the fermentation of chicken bone paste were studied using chicken bone as raw materials.The growth characteristics of lactic acid bacteria(LAB)strains,pH changes,total acidity variations,calcium distribution and cal cium-to-phosphorus ratio,composition and content of metabolites were investigated to reveal the effect of the growth of LAB on c alcium release during the fermentation of chicken bone paste.Principal component analysis(PCA),correlation ana lysis,and KEGG metabolic pathway analysis were further employed to identify the key metabolic pathways during the fe rmentation of chicken bone paste.The results showed that,LA,LR and LP strains were able to effectively utilize chick en bone paste for growth and proliferation,and the maximum viable cell count was observed at 30 h during the fermentati on processing.The total acidity contents of LA,LP and LR groups were 5.60,3.76,and 3.75 g/L respectively,significantly higher total acidity content was shown in LA group compared to other treatment groups(P<0.05).Calcium release a nalysis revealed that the total calcium content increased from 181.33 mg/kg of control group to 1176.67 mg/kg of LR,1310.00 mg/kg of LP,and 1916.67 mg/kg of LA,respectively.The free calcium content of LA,LP and LR grou ps was 40.60,50.19,and 74.62 fold-time higher than that of control group,and the free calcium content of LA group was significantly higher compared to LP and LR groups(P<0.05).X-ray diffraction and infrared spectroscopy results indicated that hydroxyapatite of bone paste mainly existed in an amorphous form.The intensity of characteristic peaks of hydroxyapatite at 2926 and 1050 cm^(-1) significantly decreased among LR,LP and LA groups compared to control group.LC-MS/MS analysis demonstrated that 37 metabolites were identified in the fermented bone paste.Principal Component An alysis(PCA)and correl

关 键 词：鸡骨泥 乳酸菌 钙释放 代谢组学 代谢物 

分 类 号：TS251.94[轻工技术与工程—农产品加工及贮藏工程]