石花菜醇提物成分分析  

Analysis of the Constituents of the Ethanol Extract of Gelidium amansii

作　　者：田永奇 罗海涛 丁婷 王奇升 邓尚贵 汪少芸[1] Tian Yongqi;Luo Haitao;Ding Ting;Wang Qisheng;Deng Shanggui;Wang Shaoyun(College of Biological Science and Engineering,Fuzhou University,Fuzhou 350108;College of Food and Pharmacy,Zhejiang Ocean University,Zhoushan 316022,Zhejiang)

机构地区：[1]福州大学生物科学与工程学院,福州350108 [2]浙江海洋大学食品与药学学院,浙江舟山316022

出　　处：《中国食品学报》2024年第2期269-280,共12页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家重点研发计划项目(2020YFD0900900)。

摘　　要：目的:研究石花菜的成分,为其功能食品的研发提供基础数据。方法:采用薄层色谱、液-质谱联用方法分析石花菜醇提物的化学成分的丰富度。通过硅胶柱、高效液相等色谱方法对其进行分离纯化,并用气-质谱联用、核磁结合文献确证化合物结构;并对部分化合物抗氧化活性进行测定。结果:薄层色谱、液-质谱联用分析表明石花菜醇提物中含有丰富的次级代谢产物,且很可能是甾醇类成分。采用气-质谱联用方法从中鉴定到29个化合物(5个为甾醇类化合物),其中棕榈酸(46.76%)、棕榈酸乙酯(13.82%)、胆甾醇(7.20%)、胆甾-4,6-二烯(4.99%)为含量最高的4个化合物。经色谱分离、核磁鉴定出9个单体化合物,分别为胆甾醇(1)、1,2-二醇植烯(2)、帕格甾醇C(3)、6-羟基-胆甾-4-烯-3-酮(4)、胆甾-5-烯-7-酮(5)、胆甾-5-烯-3β,7α二醇(6)、胆甾-5-烯-3β,7β二醇(7)、胆甾-4-烯-6-酮(8)、3-羟乙基-2-甲基-马来酰亚胺(9)。其中,化合物7清除DPPH自由基和ABTS自由基能力最好,其IC_(50)值分别为180μg/mL和12.2μg/mL。结论:首次系统研究了石花菜醇提物的成分,化合物3、8、9为新天然产物。部分甾醇类化合物具有较好的抗氧化活性。Objective:To study the chemical constituents of Gelidium amansii and lay a foundation for the research and development of functional food of Gelidium amansii.Methods:TLC and LC-MS were used to analyze the richness of chemical components in the ethanol extract of Gelidium amansii.The ethanol extract of Gelidium amansii was separated and purified by silica gel column and HPLC,and the structure of compounds were determined by GC-MS,NMR,and combined with the data reported in the literature.The antioxidant activity of some compounds was determined.Results:TLC and LC-MS analysis showed that the ethanol extract of Gelidium amansii was rich in secondary metabolites,and it was likely to be sterols.29 compounds(5 sterols)were identified from the ethanol extract by GC-MS.Among them,palmitic acid(46.76%),ethyl palmitate(13.82%),cholesterol(7.20%),4,6-cholestadien-3beta ol(4.99%)were the four compounds with the highest content.After chromatographic separation and NMR identification,9 compounds were obtained,which were cholesterol(1),1,2-diol phytoene(2),pagosterol C(3),6-hydroxy-cholest-4-en-3-one(4),cholest-5-en-7-one(5),cholest-5-ene-3β,7αdiol(6),cholest-5-ene-3β,7βdiol(7),cholest-4-en-6-one(8),3-hydroxyethyl-2-methyl-maleimide(9).Compound 7 has the strongest ability to scavenge DPPH and ABTS free radical with IC_(50) values of 0.18 mg/mL and 12.2μg/mL,respectively.Conclusion:In this paper,the ethanol extract of Gelidium amansii were systematically studied for the first time.Compounds 3,8 and 9 were new natural products.Some sterols showed good antioxidant activity.

关 键 词：石花菜醇提物 成分 甾体 抗氧化活性 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.63[轻工技术与工程—食品科学与工程]