响应面法优化花生水酶法制油水相体系中残油工艺  

Optimization of aqueous enzymatic process of peanut with residual oil in aqueous phase by response surface methodology

作　　者：熊浩然 郭兴凤[1] 郭倩倩[1] 田亚东 朱婷伟[1] 陈复生[1] XIONG Haoran;GUO Xingfeng;GUO Qianqian;TIAN Yadong;ZHU Tingwei;CHEN Fusheng(College of Food Science and Engineering,Henan University of Technology,Zhengzhou 450001,China)

机构地区：[1]河南工业大学粮油食品学院,河南郑州450001

出　　处：《河南工业大学学报（自然科学版）》2024年第1期57-63,共7页Journal of Henan University of Technology：Natural Science Edition

基　　金：国家自然科学基金区域创新联合基金重点项目(U21A20270)。

摘　　要：水酶法可以实现花生油脂和蛋白的同步提取,但得到的水相体系中部分油脂稳定存在,不利于油脂得率的提升。通过湿法粉碎花生,利用激光共聚焦显微镜(CLSM)和生物显微镜对花生水酶法制油水相体系的微观形态进行观察,分析不同粉碎时间下水相残油率和油滴分布之间的联系。在单因素试验基础上,采用响应面法优化影响水相残油率的工艺条件。结果表明:花生水酶法制油得到的水相是一个高蛋白低油脂的体系,当粉碎时间为120 s时,水相残油率最低(10.78%),水相体系油滴分布最少;最佳工艺条件为酶解时间1.37 h、酶解温度47.92℃、加酶量1.25%、液料比4.89∶1 mL/g,在此条件下得到的水相残油率为8.79%±0.03%,与响应面预测值8.82%无显著性差异。研究结果为间接提高水酶法制油提升油脂得率提供理论依据。Aqueous enzymatic process is a technology for extracting peanut oil and protein simultaneously.However,peanut oil dispersing in aqueous phase stably is not beneficial for the improvement of the oil yield.In this study,the micromorphology of the aqueous phase system was observed by CLSM and biomicroscope through wet grinding of peanuts,and the relationship between aqueous phase oil residual rate and oil droplet distribution were analyzed under different grinding time.The results showed that the aqueous phase obtained by aqueous enzymatic process was a high protein and low oil content system.When the grinding time was 120 s,the lowest aqueous phase residual oil rate was obtained and the content was 10.78%,and the oil droplets in the aqueous phase had the smallest number.Single factor experiment was designed to study the effect of enzymatic hydrolysis conditions on the aqueous phase residual oil rate,and the optimal conditions were obtained through response surface methodology.The optimal conditions were as follows:enzymatic hydrolysis time 1.37 h,temperature 47.92℃,enzyme concentration 1.25%,and liq-uid-solid ratio 4.89∶1 mL/g.Under these conditions,the aqueous phase residual oil rate was 8.79%±0.03%,which had no significant difference with the predicted value of 8.82%.This result would provide a theoretical basis for the improvement of oil yield by aqueous enzymatic process.

关 键 词：水酶法 水相体系 微观形态 残油率 响应面法 

分 类 号：TS201.2[轻工技术与工程—食品科学]