一株源自传统大豆发酵食品中纳豆菌株的分离鉴定与分析  

Isolation,Identification and Analysis of a Natto Strain from Traditional Fermented Soybean Food

作　　者：徐林通 张冬鹤 侯进慧[1] 李宁宁 谈晟含 文宇 XU Lintong;ZHANG Donghe;HOU Jinhui;LI Ningning;TAN Shenghan;WEN Yu(Xuzhou University of Technology,Xuzhou,Jiangsu 221018,China)

机构地区：[1]徐州工程学院,江苏徐州221018

出　　处：《农产品加工》2024年第4期59-62,共4页Farm Products Processing

基　　金：江苏省第十五批“六大人才高峰”高层次人才项目(SWYY-230);江苏省科技厅苏北科技专项科技富民强县项目(XZ-SZ201930);江苏省高等学校大学生创新创业训练计划项目(202111998021)。

摘　　要：从我国传统大豆发酵食品中分离获得多株菌株,用划线纯培养法对其中1株纳豆激酶活性较高的菌株SD1进行生理生化和16S rDNA分析,并分析其纳豆激酶基因,推动新型纳豆菌开发。结果表明,菌落呈现白色,表面干燥,边缘有褶皱,中心无突起,整体平滑,呈现出芽胞杆菌的菌落特征。提取菌株SD1的基因组DNA作为模板,扩增其16S rDNA分子序列,利用凝胶电泳进行检验、测序。对获得的序列进行BLAST分析显示,SD1菌株是枯草芽孢杆菌(Bacillus subtilis),采用设计引物,可在该菌中扩增出特异性的纳豆激酶目的条带,结果符合预期。将扩增条带寄送测序,获得序列长度为1174 bp,将对应的氨基酸序列在SWISS网站上在线分析其三维结构;在预测三维结构中,含有6个α-螺旋,12个β-折叠。为新型纳豆菌的开发和利用提供基础。Several strains were isolated from traditional soybean fermented food.Strain SD1 with high nattokinase activity was analyzed by physiological and biochemical analysis and 16S rDNA analysis.Nattokinase gene of the strain was analyzed to promote the development of a new type of nattokinase.The results showed that the colony was white,with dry surface,wrinkles on the edge,no protrusion in the center,and was smooth as a whole,showing the characteristics of the colony of Bacillus.The genomic DNA of strain SD1 was extracted as a template,and its 16S rDNA molecular sequence was amplified,tested by gel electrophoresis,and sent to sequencing.BLAST analysis of the obtained sequence showed that SD1 strain was Bacillus subtilis.Specific nattokinase target bands could be amplified in the strain.The amplified bands were sent for sequencing,and the length of the sequence was 1174 bp.The corresponding amino acid sequence was online analyzed on SWISS website for its three-dimensional structure.In the predicted three-dimensional structure,there were 6α-helix,12β-sheet.This study provided a basis for the development and utilization of new natto bacteria.

关 键 词：纳豆菌 菌种鉴定 纳豆激酶基因 序列分析 

分 类 号：Q939[生物学—微生物学]