Biomaterial engineering strategies for modeling the Bruch's membrane in age-related macular degeneration  

作　　者：Blanca Molins Andrea Rodríguez Víctor Llorenç Alfredo Adán 

机构地区：[1]Group of Ocular Inflammation:Clinical and Experimental Studies,Institut d’Investigacions Biomèdiques AgustíPi I Sunyer(IDIBAPS),Barcelona,Spain [2]Institut Clínic d’Oftalmologia(ICOF),Hospital Clínic Barcelona,Spain

出　　处：《Neural Regeneration Research》2024年第12期2626-2636,共11页中国神经再生研究（英文版）

基　　金：supported by the Ministry of Science and Innovation of Spain,"Instituto de Salud CarlosⅢ","Fon do de Investigacion Sanitaria" (PI19/00265);funds FEDER"Una manera de hacer Europa" (to BM)。

摘　　要：Age-related macular degeneration,a multifactorial inflammatory degenerative retinal disease,ranks as the leading cause of blindness in the elderly.Strikingly,there is a scarcity of curative therapies,especially for the atrophic advanced form of age-related macular degeneration,likely due to the lack of models able to fully recapitulate the native structure of the outer blood retinal barrier,the prime to rget tissue of age-related macular degeneration.Standard in vitro systems rely on 2D monocultures unable to adequately reproduce the structure and function of the outer blood retinal barrier,integrated by the dynamic interaction of the retinal pigment epithelium,the Bruch's membrane,and the underlying choriocapillaris.The Bruch's membrane provides structu ral and mechanical support and regulates the molecular trafficking in the outer blood retinal barrier,and therefo re adequate Bruch's membrane-mimics are key for the development of physiologically relevant models of the outer blood retinal barrie r.In the last years,advances in the field of biomaterial engineering have provided novel approaches to mimic the Bruch's membrane from a variety of materials.This review provides a discussion of the integrated properties and function of outer blood retinal barrier components in healt hy and age-related macular degeneration status to understand the requirements to adequately fabricate Bruch's membrane biomimetic systems.Then,we discuss novel materials and techniques to fabricate Bruch's membrane-like scaffolds for age-related macular degeneration in vitro modeling,discussing their advantages and challenges with a special focus on the potential of Bruch's membrane-like mimics based on decellularized tissue.

关 键 词：age-related macular degeneration Bruch's membrane DECELLULARIZATION retinal pigment epithelium SCAFFOLD 

分 类 号：R774.5[医药卫生—眼科]