浓缩苹果汁中嗜酸耐热菌的分离鉴定及菌群特性研究  

Isolation,Identification and the Bacteria Colony Characteristic of Thermo-acidophilic Bacteria in the Apple Juice Concentrate

作　　者：孟秀芹 丁建姿 张辉 王坤 吴茂玉[4] 申文斌 赵岩[4] 初乐[4] MENG Xiuqin;DING Jianzi;ZHANG Hui;WANG Kun;WU Maoyu;SHEN Wenbin;ZHAO Yan;CHU Le(Yantai North Andre Juice Co.,Ltd.,Yantai 264100,China;Key Laboratory of Apple Deep Processing Technology of Shandong Province,Yantai 264100,China;Fruit and Vegetable Storage and Processing Technology Innovation Center of Shandong Province,Yantai 264100,China;Jinan Fruit Research Institute,All China Federation of Supply&Marketing Cooperatives,Jinan 250014,China)

机构地区：[1]烟台北方安德利果汁股份有限公司,山东烟台264100 [2]山东省苹果精深加工技术重点实验室,山东烟台264100 [3]山东省果蔬贮藏加工技术创新中心,山东烟台264100 [4]中华全国供销合作总社济南果品研究所,山东济南250014

出　　处：《中国果菜》2024年第3期28-33,共6页China Fruit & Vegetable

基　　金：山东省重点研发计划(重大科技创新工程)(2022CXGC010601)。

摘　　要：为了解和控制浓缩苹果汁中的嗜酸耐热菌,从胀袋浓缩苹果汁中筛选、分离、纯化得到了1株嗜酸耐热菌,并进行菌种鉴定及特性研究。通过菌落形态、显微形态和16S rDNA基因测序以及建立系统发育树分析,初步确定该分离菌与Aiyclobacillus acidoterrestris ATCC 49025标准菌同源度>99.51%,确定该分离菌为酸土脂环酸芽孢杆菌(A.acidoterrestris)。通过热和酸碱处理考察该菌株的热稳定性和酸碱稳定性,在单因素试验的基础上进行正交试验。结果表明,在最优灭菌参数为pH 6.0,105℃热处理20 min的条件下,平板生长菌落数为0,说明该提取条件具有较好的合理性和可靠性,为浓缩苹果汁中嗜酸耐热菌的控制提供了理论依据。In order to understand and control thermo-acidophilic bacteria in apple juice concentrate,a strain of thermo-acidophilic bacteria was screened,isolated,and purified from the apple juice concentrate in expansion bags.The thermo-acidophilic bacteria were identified by cell morphology,colony morphology,16S rDNA test with phylogenetic tree analysis.The result showed that the homology of isolated strain and standard bacteria ATCC 49025 reached to 99.51%.The isolated strain was identified as Aiyclobacillus acidoterrestris.The thermal stability and acid-base stability of the strain were examined by heat and acid-base treatment.Orthogonal experiment was carried out on the base of single factor experiments,and it was found that the optimal sterilization parameter was to heat treat at 105℃for 20 min at pH 6.0.Under this parameter,the number of colonies growing on the plate was 0.The result indicated that the extraction conditions have good rationality and reliability.The conclusion provided a theoretical basis for the control of thermo-acidophilic bacteria in apple juice concentrate.

关 键 词：浓缩苹果汁 筛选分离 嗜酸耐热菌 分离鉴定 菌群特性 

分 类 号：TS255.44[轻工技术与工程—农产品加工及贮藏工程]