利用竹基纤维素为碳源生物转化L-乳酸的潜力研究  

Study on potential of bioconversion of L-lactic acid using bamboo-based cellulose as carbon source

作　　者：杨春柳 管秀琼 刘春 何明雄 刘林培 YANG Chunliu;GUAN Xiuqiong;LIU Chun;HE Mingxiong;LIU Linpei(School of Biological Engineering,Sichuan Uniersity of Science&Engineering,Zigong 643000,China;Biomass Energy Technology Research Centre,Key Laboratory of Development and Application of Rural Renewable Energy(Ministry of Agriculture and Rural Affairs),Biogas Institute of Ministry of Agriculture and Rural Affairs,Chengdu 610041,China)

机构地区：[1]四川轻化工大学生物工程学院,四川自贡643000 [2]农业农村部沼气科学研究所,农业农村部可再生能源开发与利用重点实验室,生物质能技术研究中心,四川成都610041

出　　处：《食品与发酵工业》2024年第6期177-183,共7页Food and Fermentation Industries

基　　金：四川省科技厅重点研发项目(2023YFN0025);四川省科技成果转移转化项目(2021ZHZY0015)。

摘　　要：以竹材制浆厂备料工段产生的废弃竹屑为原料,采用蒸汽爆破预处理且提取半纤维素后的竹基纤维素为碳源,同时对预处理前后竹屑采用扫描电子显微镜&能谱仪和激光粒度分析仪进行表征;用分步水解发酵工艺,结合高效液相色谱对发酵L-乳酸工艺关键参数进行了分析,探究了该碳源生物转化L-乳酸的潜力。结果表明,蒸汽爆破预处理提高了原料酶解还原糖释放量,在总固体(total solids,TS)质量浓度140 g/L、酶添加量60 FPU/g、酶解54 h条件下糖化,总糖质量浓度可达31.19 g/L。将糖化液用于发酵试验,在起始糖质量浓度19.43 g/L,发酵初始pH 6.5、菌株接种量5%、发酵17 h条件下,L-乳酸质量浓度可达6.28 g/L,其糖酸转化率高达80.87%。综上,该实验为竹基纤维素的利用提供了高值化途径参考。Using the waste bamboo sawdust produced in the material preparation section of bamboo pulping plant as raw materials,bamboo-based cellulose after steam explosion pretreatment and extraction of hemicelluloses was used as carbon source.The bamboo sawdust before and after pretreatment were characterized by scanning electron microscopy&energy dispersive spectrometer and laser particle size analyzer.The key parameters of the fermentation process of L-lactic acid were analyzed by step hydrolysis fermentation and high performance liquid chromatography,and the potential of bioconversion of L-lactic acid from the carbon source was investigated.Results showed that steam explosion pretreatment increased the release of reducing sugar from the raw material,and the total sugar concentration reached 31.19 g/L under the conditions of 140 g/L of total solid concentration,60 FPU/g of enzyme addition,and enzymatic hydrolysis for 54 h.The saccharification solution was used in the fermentation test.Under the conditions of initial sugar concentration of 19.43 g/L,initial pH 6.5,inoculation amount of 5%,and fermentation for 17 h,the L-lactic acid concentration could reach 6.28 g/L,and the saccharic acid conversion rate was as high as 80.87%.In conclusion,the experiment provides a reference for the utilization of bamboo-based cellulose.

关 键 词：L-乳酸 竹屑 蒸汽爆破预处理 分步水解发酵 纤维素 

分 类 号：TQ921.3[轻工技术与工程—发酵工程]