基于RBL-2H3细胞脱颗粒抑制作用的体外抗过敏活性益生菌的筛选及鉴定  

Screening and Identification of Probiotics with in Vitro Anti-Allergic Activity Based on the Degranulation Inhibition of RBL-2H3 Cells

作　　者：马丁 秦双霞 郝慧超 邓放明[1,2] 赵玲艳[1,2] MA Ding;QIN Shuangxia;HAO Huichao;DENG Fangming;ZHAO Lingyan(College of Food Science and Technology,Hunan Agricultural University,Changsha 410128,China;Changsha Innovation Institute for Food,Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]湖南农业大学食品科学技术学院,湖南长沙410128 [2]湖南农业大学长沙现代食品创新研究院,湖南长沙410128

出　　处：《食品科学》2024年第6期55-63,共9页Food Science

基　　金：国家现代农业产业技术体系建设专项(CARS-24-E-02);湖南农业大学“双一流”建设项目(SYL20182006);植物源益生菌菌种资源库建设及开发应用研究项目(AU-YJY-B-LX-20-014);湖南省自然科学基金面上项目(2021JJ30338);发酵辣椒绿色精深加工工程技术研究中心建设项目(2023ZYQ044)。

摘　　要：为探究乳酸菌的抗过敏活性,本研究采用传统分离技术从发酵蔬菜中获得乳酸菌,并进行益生菌体外评价,结合RBL-2H3细胞脱颗粒抑制实验筛选具有抗过敏活性的益生菌。结果表明,从发酵蔬菜中分离的4株菌株表现出良好的益生特性和安全性;所选4株乳酸菌对RBL-2H3细胞脱颗粒释放β-己糖胺酶均具有较高的抑制作用,并对RBL-2H3细胞脱颗粒释放组胺、肿瘤坏死因子-α和白介素-4均有明显的下调作用,具有作为抗过敏活性益生菌的可能性。4株菌株经16S rDNA测序鉴定为植物乳杆菌2株、发酵乳杆菌和副干酪乳杆菌各1株。To investigate the anti-allergic activity of lactic acid bacteria,lactic acid bacterial strains were obtained from fermented vegetables by the traditional isolation technique and screened for in vitro allergic activity by the degranulation inhibition assay against RBL-2H3 cells.The results showed that all four isolates exhibited good probiotic properties and safety and had a strong inhibitory effect onβ-hexosaminidase released from RBL-2H3 cells after degranulation.Moreover,they showed significant down-regulation effects on the release of histamine,tumor necrosis factor-αand interleukin-4 from RBL-2H3 cell after degranulation,suggesting their potential as anti-allergic probiotics.The four strains were identified by 16S rDNA sequencing as two strains of Lactobacillus plantarum,one strain of L.fermentum and one strain of L.paracasei.

关 键 词：乳酸菌 RBL-2H3细胞 益生特性 脱颗粒 抗过敏活性 

分 类 号：TS201.3[轻工技术与工程—食品科学]