表没食子儿茶素没食子酸酯与L-茶氨酸对乙醇脱氢酶和乙醛脱氢酶活性的体外协同作用  

Synergistic effect of epigallocatechin gallate and L-theanine on alcohol dehydrogenase and acet aldehyde dehydrogenase in vitro

作　　者：杨悦 刘梦圆 肖文军[1,2] YANG Yue;LIU Meng-Yuan;XIAO Wen-Jun(Key Lab of Tea Science of Ministry of Education,Hunan Agricultural University,Changsha 410128,China;National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients,Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]湖南农业大学茶学教育部重点实验室,长沙410128 [2]湖南农业大学国家植物功能成分利用工程技术研究中心,长沙410128

出　　处：《食品安全质量检测学报》2024年第4期260-265,共6页Journal of Food Safety and Quality

基　　金：国家重点研发计划项目(2022YFD1600805);湖南省科技重大项目(2021NK1020)。

摘　　要：目的探究表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG)与L-茶氨酸对乙醇脱氢酶(alcohol dehydrogenase,ADH)和乙醛脱氢酶(acetaldehyde dehydrogenase,ALDH)活性的体外协同作用。方法设置不同浓度及组分,以0μg/mL实验组为对照,先后进行单因素实验和最优组合验证实验,通过对ADH、ALDH活性的检测,确认EGCG、L-茶氨酸不同剂量的解酒效果并探究其最佳配比。结果单因素实验中,样品质量浓度为200、300μg/mL实验组相较于0μg/mL浓度组,随着EGCG和L-茶氨酸的添加,ADH和ALDH活性显著高于空白组(P<0.05);最优组合验证实验中,11组不同配比组合物对ADH和ALDH活性均具有促进作用,且协同组比单因素组激活效果要更好。其中,当配比组合物为7:3时,ADH、ALDH的活性最高。结论EGCG、L-茶氨酸解酒组合物最佳配比为7:3时ADH与ALDH活性最高,研究结果为EGCG、L-茶氨酸组合物用于抗醉酒药物研发提供科学依据。Objective To explore the synergistic effect of epigallocatechin gallate(EGCG)and L-theaninein on alcohol dehydrogenasee(ADH)and acetaldehyde dehydrogenasee(ALDH)in vitro.Methods Different concentrations and compositions were set,with the experimental group at Oμg/mL serving as the control.Single-factor experiments and combination verification experiments were conducted.Through the detection of ADH and ALDH activities,the sobering effects of EGCG and L-theanine were confirmed,and their optimal ratio was explored.Results In the single-factor experiment,compared to the Oμg/mL concentration group,the experimental group with a sample mass concentration of 200μg/mL,300μg/mL showed a significant increase in ADH and ALDH activities than those of the blank group with the addition of EGCG and L-theanine(P<0.05).In the optimal combination verification experiment,all 1l different ratio combinations exhibited a promoting effect on both ADH and ALDH activities,with the synergistic group showing a better activation effect compared to the single-factor groups.Specifically,when the ratio of the combination was 7:3,the activities of ADH and ALDH were the highest.Conclusion The optimal ratio of EGCG to L-theanine for the anti-intoxication effect is 7:3,resulting in the highest activities of ADH and ALDH.The findings provide scientific evidence for the development of drugs for anti-intoxication using EGCG and L-theanine combination.

关 键 词：表没食子儿茶素没食子酸酯 L-茶氨酸 乙醇脱氢酶 乙醛脱氢酶 协同作用 

分 类 号：TQ460.1[化学工程—制药化工]