葡萄糖氧化酶和植物乳杆菌抑菌性能的研究  

Antibacterial activity of glucose oxidase and Lactobacillus plantarum

作　　者：谢全喜[1] 陈雷 徐海燕[1] 辛国芹[1] 侯楠楠[1] 谷巍[1] XIE Quanxi;CHEN Lei;XU Haiyan;XIN Guoqin;HOU Nannan;GU Wei(Shandong BaoLai-LeeLai Bioengineering Co.,Ltd.,Shandong Key Laboratory of Animal Microecological Preparations,Taian 271000,China;Taishan Polytechnic,Taian 271000,China)

机构地区：[1]山东宝来利来生物工程股份有限公司山东省动物微生态制剂重点实验室,山东泰安271000 [2]泰山职业技术学院,山东泰安271000

出　　处：《中国酿造》2024年第3期132-135,共4页China Brewing

基　　金：泰安市科技发展计划(201540701)。

摘　　要：采用牛津杯法研究葡萄糖氧化酶(GOD)和植物乳杆菌(Lactobacillus plantarum)对鸡源、猪源大肠杆菌(Escherichia coli)和沙门氏菌(Salmonella)的抑菌作用。结果表明,牛津杯法检测到GOD对不同来源致病菌均具有抑菌性,且在一定浓度范围内随浓度增加抑菌性能增强,在GOD酶活10 U/mL时仍具有抑菌作用,抑菌圈直径均≥9.25 mm。GOD酶在37~75℃干热处理10 min后仍具有较好的抑菌活性,65℃时对猪源大肠杆菌K88和37℃时对沙门氏菌0533抑菌作用最强,抑菌圈直径分别为10.75 mm和12.25 mm。植物乳杆菌BLCC2-0126对被试病原菌抑菌圈直径最大,对猪源大肠杆菌K99和沙门氏菌0533抑菌圈直径为13.75 mm。总之,GOD和BLCC2-0126均具有抑菌作用,且可共用于实际生产中。The antibacterial effects of glucose oxidase(GOD)and Lactobacillus plantarum on Escherichia coli and Salmonella from chickens and pigs were determined by Oxford cup method.The results showed that GOD had antibacterial activity against pathogenic bacteria from different sources detected by the Oxford Cup method,and its antibacterial activity increased with increasing concentration within a certain concentration range.It still had antibacterial activity at enzyme concentration of 10 U/ml,and the diameter of the antibacterial zone were all≥9.25 mm.GOD enzyme still had good antibacterial activity after 10 min dry heat treatment at 37-75℃.The strongest antibacterial activity was observed against E.coli K88 from pig at 65℃and Salmonella 0533 at 37℃,with inhibitory zone diameters of 10.75 mm and 12.25 mm,respectively.The diameter of the inhibitory zone of L.plantarum BLCC2-0126 against the tested pathogenic bacteria was the largest for tested pathogens,while the diameter of the inhibitory zone against E.coli K99 and Salmonella 0533 from pig was 13.75 mm.In summary,both GOD and BLCC2-0126 had antibacterial effects and could be used together in practical production.

关 键 词：葡萄糖氧化酶 植物乳杆菌 牛津杯法 抑菌作用 

分 类 号：TS255.54[轻工技术与工程—农产品加工及贮藏工程]