响应面分析优化晋西北酸粥和糜米总酚的提取工艺  

Optimization of Extraction Process of Total Phenols from Sour Porridge in Northwestern Shanxi and Milled Nonglutinous Broomcorn Millet by Response Surface Analysis

作　　者：王琪[1] 彭佳伟 WANG Qi;PENG Jia-wei(School of Life Science,Shanxi University,Taiyuan 030031,China)

机构地区：[1]山西大学生命科学学院,太原030031

出　　处：《中国调味品》2024年第4期127-133,共7页China Condiment

基　　金：山西省自然科学基金面上项目(20210302123464)。

摘　　要：以总酚提取量为指标,研究自然发酵的晋西北酸粥和其原材料糜米中总酚的最佳提取工艺,采用福林酚比色法测定总酚提取量。比较不同的单因素对晋西北酸粥和糜米中总酚提取量的影响。在此基础上,通过响应面分析,探讨不同丙酮浓度、料液比、提取时间和提取温度对总酚提取量的影响。结果发现,晋西北酸粥中总酚的最佳提取条件为丙酮浓度58.06%、料液比1∶41.13、提取时间30.57 min和提取温度41.81℃,在此条件下总酚的最大提取量为1.88 mg/g。糜米中总酚的最佳提取条件为丙酮浓度84.78%、料液比1∶44.76、提取时间34.34 min、提取温度42.11℃,在此条件下总酚的最大提取量为1.51 mg/g,而且晋西北酸粥中总酚提取量约是糜米中总酚提取量的1.24倍。The optimal extraction process of total phenols from naturally fermented sour porridge in northwestern Shanxi and its raw material milled nonglutinous broomcorn millet is studied with the extraction amount of total phenols as the index,and the extraction amount of total phenols is determined by Folin-Ciocalteu colorimetry.The effects of different single factors on the extraction amount of total phenols from sour porridge in northwestern Shanxi and milled nonglutinous broomcorn millet are compared.On this basis,the effects of different acetone concentrations,solid-liquid ratios,extraction time and extraction temperatures on the extraction amount of total phenols are investigated by response surface analysis.The results show that the optimal extraction conditions of total phenols from sour porridge in northwestern Shanxi are as follows:acetone concentration is 58.06%,solid-liquid ratio is 1∶41.13,extraction time is 30.57 min and extraction temperature is 41.81℃.Under these conditions,the maximum extraction amount of total phenols is 1.88 mg/g.The optimal extraction conditions of total phenols from milled nonglutinous broomcorn millet are as follows:acetone concentration is 84.78%,solid-liquid ratio is 1∶44.76,extraction time is 34.34 min and extraction temperature is 42.11℃.Under these conditions,the maximum extraction amount of total phenols is 1.51 mg/g,and the extraction amount of total phenols from sour porridge in northwestern Shanxi is about 1.24 times that of milled nonglutinous broomcorn millet.

关 键 词：晋西北酸粥 糜米 总酚 响应面实验优化 

分 类 号：TS201.2[轻工技术与工程—食品科学]