Washed microbiota transplantation for Crohn’s disease:A metagenomic,metatranscriptomic,and metabolomic-based study  

作　　者：Shi-Ju Chen Da-Ya Zhang Xia Wu Fa-Ming Zhang Bo-Ta Cui Yi-Hao Huang Zu-Lun Zhang Rui Wang Fei-Hu Bai 

机构地区：[1]Graduate School,Hainan Medical University,Haikou 571199,Hainan Province,China [2]Department of Microbiota Medicine&Medical Center for Digestive Diseases,The Second Affiliated Hospital of Nanjing Medical University,Nanjing 210011,Jiangsu Province,China [3]Department of Gastroenterology,The Second Affiliated Hospital of Hainan Medical University,Haikou 570216,Hainan Province,China

出　　处：《World Journal of Gastroenterology》2024年第11期1572-1587,共16页世界胃肠病学杂志（英文版）

基　　金：Supported by the Innovation Platform for Academicians of Hainan Province,No.YSPTZX202313;Hainan Province Clinical Medical Center,No.2021818;Hainan Provincial Health Industry Research Project,No.22A200078;Hainan Provincial Postgraduate Innovation Research Project,No.Qhyb2022-133;Hainan Medical University Graduate Student Innovative Research Project,No.HYYB2022A18;Nanjing Medical University Fan Daiming Research Funds for Holistic Integrative Medicine,No.2020-3HIM.

摘　　要：BACKGROUND Fecal microbiota transplantation(FMT)is a promising therapeutic approach for treating Crohn’s disease(CD).The new method of FMT,based on the automatic washing process,was named as washed microbiota transplantation(WMT).Most existing studies have focused on observing the clinical phenomena.However,the mechanism of action of FMT for the effective management of CD-particularly in-depth multi-omics analysis involving the metagenome,metatranscriptome,and metabolome-has not yet been reported.AIM To assess the efficacy of WMT for CD and explore alterations in the microbiome and metabolome in response to WMT.METHODS We conducted a prospective,open-label,single-center clinical study.Eleven CD patients underwent WMT.Their clinical responses(defined as a decrease in their CD Activity Index score of>100 points)and their microbiome(metagenome,metatranscriptome)and metabolome profiles were evaluated three months after the procedure.RESULTS Seven of the 11 patients(63.6%)showed an optimal clinical response three months post-WMT.Gut microbiome diversity significantly increased after WMT,consistent with improved clinical symptoms.Comparison of the metagenome and metatranscriptome analyses revealed consistent alterations in certain strains,such as Faecalibac-terium prausnitzii,Roseburia intestinalis,and Escherichia coli.In addition,metabolomics analyses demonstrated that CD patients had elevated levels of various amino acids before treatment compared to the donors.However,levels of vital amino acids that may be associated with disease progression(e.g.,L-glutamic acid,gamma-glutamyl-leucine,and prolyl-glutamine)were reduced after WMT.CONCLUSION WMT demonstrated therapeutic efficacy in CD treatment,likely due to the effective reconstruction of the patient’s microbiome.Multi-omics techniques can effectively help decipher the potential mechanisms of WMT in treating CD.

关 键 词：Crohn’s disease Clinical trials Fecal microbiota transplant METAGENOME Metatranscriptome METABOLOME 

分 类 号：R571[医药卫生—消化系统]