姜黄素联合富硒酵母对小鼠急性酒精性肝损伤的保护作用  

Protective Effect of Curcumin and Selenium-Rich Yeast on Acute Alcoholic Hepatic Injury in Mice

作　　者：梁婵华 莫丽 田重阳 唐玉涵[1] LIANG Chanhua;MO Li;TIAN Chongyang;TANG Yuhan(School of Public Health,Tongji Medical College,Wuhan 430030,China)

机构地区：[1]华中科技大学同济医学院公共卫生学院,湖北武汉430030

出　　处：《食品安全导刊》2024年第9期48-53,共6页China Food Safety Magazine

摘　　要：目的:探讨姜黄素联合富硒酵母对急性酒精性肝损伤的保护作用。方法:将60只C57B6L/J小鼠随机分为空白组、模型组、姜黄素组(33.33 mg·kg^(-1) bw)、富硒酵母组(7.50 mg·kg^(-1) bw)和联合组(姜黄素33.33 mg·kg^(-1) bw+富硒酵母7.50 mg·kg^(-1) bw),每组12只;干预30 d后,一次性灌胃50%乙醇溶液(12 mL·kg^(-1) bw)建立急性酒精性肝损伤模型;观察小鼠体重、摄食、行为变化;检测小鼠肝脏指数与丙二醛(Malondialdehyde,MDA)、还原型谷胱甘肽(Glutathione,GSH)和甘油三酯(Triglyceride,TG)水平;油红染色观察小鼠肝脏组织病理学变化。结果:与空白组比较,模型组小鼠表现出肝脏肿大、肝细胞中脂滴大量堆积病理损伤,肝脏组织MDA和TG水平显著升高(P<0.05);姜黄素联合富硒酵母能显著降低小鼠肝脏中MDA和TG表达,提高GSH水平(P<0.05),减轻小鼠肝脏脂肪沉积。结论:姜黄素联合富硒酵母能够改善急性酒精灌胃诱导的肝脏脂质异常堆积,减轻氧化应激水平,对急性酒精性肝损伤具有良好的保护作用。Objective:Exploring the protective effect of curcumin combined with selenium-rich yeast on acute alcoholic liver injury.Method:60 C57B6L/J mice were randomly divided into blank group,model group,curcumin group(33.33 mg·kg^(-1) bw),selenium-rich yeast group(7.50 mg·kg^(-1) bw)and combination group(curcumin 33.33 mg·kg^(-1) bw+selenium-rich yeast 7.50 mg·kg^(-1) bw),12 mice in each group;30 days after the intervention,a model of acute alcoholic liver injury was established by one-time gavage 50%ethanol solution(12 mL·kg^(-1) bw);the weight,feeding and behavior changes of mice were observed;liver index and the levels of malondialdehyde(MDA),glutathione(GSH)and triglyceride(TG)were detected,and the histopathological changes of the liver were observed by oil red staining.Result:Compared to control group,the model mice showed liver enlargement,massive accumulation of lipid droplets in liver cells,and significantly increased MDA and TG levels(P<0.05)in liver tissues,while curcumin combined with selenium-rich yeast could significantly reduce the expression of MDA and TG in the liver,increase the level of GSH(P<0.05),and reduce the fat deposition in the liver.Conclusion:Curcumin combined with selenium-rich yeast can improve abnormal accumulation of liver lipids induced by acute alcohol gavage,reduce the level of oxidative stress,and have a good protective effect against acute alcoholic liver injury.

关 键 词：姜黄素 富硒酵母 急性酒精性肝损伤 

分 类 号：TS201.4[轻工技术与工程—食品科学] R575[轻工技术与工程—食品科学与工程]