冷冻猪肉食品中金黄色葡萄球菌的定性检测方法  

Qualitative Detection Method for Staphylococcus aureus in Frozen Pork Food

作　　者：慕礼霞 贺治芬 MU Lixia;HE Zhifen(Jingbian County Market Inspection and Testing Center,Yulin 718500,China;Jingbian County Market Supervision Administration,Yulin 718500,China)

机构地区：[1]靖边县市场检验检测中心,陕西榆林718500 [2]靖边县市场监督管理局,陕西榆林718500

出　　处：《食品安全导刊》2024年第9期114-117,共4页China Food Safety Magazine

摘　　要：目的:分析冷冻猪肉食品中金黄色葡萄球菌的污染情况。方法:2023年7—12月,随机抽选A市冷冻猪肉食品150份为研究样本。样本处理后分离菌株并培养,进行革兰染色和血浆凝固酶试验。观察金黄色葡萄球菌的生物学性状,统计冷冻猪肉样本中金黄色葡萄球菌的检出情况。结果:28份样本受到金黄色葡萄球菌污染,即金黄色葡萄球菌阳性检出率为18.67%。不同来源样本中,来源于农贸市场的样品阳性检出率为26.39%,高于商超的11.54%;不同类型样本中,纯肉类样品阳性检出率为30.00%,高于排骨类和脏器类的12.00%、14.00%;不同冷冻时间样本中,冷冻1、2、3个月的样品阳性检出率分别是17.74%、18.75%和20.00%。结论:农贸市场中的纯肉类冷冻猪肉食品,其金黄色葡萄球菌污染情况较严重,提示相关管理部门加强监管,以保障食品安全。Objective:To analyze the contamination of Staphylococcus aureus in frozen pork food.Method:From July to December 2023,150 frozen pork foods from city A were randomly selected as research samples.After sample processing,isolate the strains and culture them for Gram staining and plasma coagulation enzyme test.Observe the biological characteristics of Staphylococcus aureus and count the detection of Staphylococcus aureus in frozen pork samples.Result:28 samples were contaminated with Staphylococcus aureus,with a positive detection rate of 18.67%.Among samples from different sources,the positive detection rate from agricultural markets was 26.39%,higher than the 11.54%from supermarkets;in different types of samples,the positive detection rate of pure meat is 30.00%,which is higher than the 12.00%and 14.00%of pork ribs and organs;the positive detection rates for samples frozen for 1,2,and 3 months at different freezing times were 17.74%,18.75%,and 20.00%,respectively.Conclusion:The contamination of Staphylococcus aureus in pure meat frozen pork products in agricultural markets is severe,which suggests that relevant management departments should strengthen supervision to ensure food safety.

关 键 词：冷冻猪肉 金黄色葡萄球菌 分离培养 定性检测 市场监管 

分 类 号：R155.5[医药卫生—营养与食品卫生学]