X-box binding protein 1 caused an imbalance in pyroptosis and mitophagy in immature rats with di-(2-ethylhexyl)phthalate-induced testis toxicity  

作　　者：Yifan Hong Xiazhu Zhou Qi Li Jing Chen Yuexin Wei Chunlan Long Lianju Shen Xiangqin Zheng Dinggang Li Xia Wang Chenjun Yu Shengde Wu Guanghui Wei 

机构地区：[1]Department of Urology,Children's Hospital of Chongqing Medical University,Chongqing 400014,China [2]Pediatric Research Institute,Children's Hospital of Chongqing Medical University,Chongqing 400014,China [3]Chongqing Key Laboratory of Children Urogenital Development and Tissue Engineering,Children's Hospital of Chongqing Medical University,Chongqing 400014,China [4]Chongqing Key Laboratory of Pediatrics,Children's Hospital of Chongqing Medical University,Chongqing 400014,China [5]Ministry of Education Key Laboratory of Child Development and Disorders,Children's Hospital of Chongqing Medical University,Chongqing 400014,China [6]National Clinical Research Center for Child Health and Disorders,Children's Hospital of Chongqing Medical University,Chongqing 400014,China [7]China International Science and Technology Cooperation Base of Child Development and Critical Disorders,Children's Hospital of Chongqing Medical University,Chongqing 400014,China

出　　处：《Genes & Diseases》2024年第2期935-951,共17页基因与疾病（英文）

基　　金：supported by the National Natural Science Foundation of China(No.81771566,82071632);the Postgraduate Research Innovation Project of Chongqing Medical University(China)(No.CYB22210);the Youth Basic Research Project from the Ministry of Education Key Laboratory of Child Development and Disorders(China)(No.YBRP-202114).

摘　　要：As a widely used plasticizer,di-(2-ethylhexyl)phthalate(DEHP)is known to induce significant testicular injury.However,the potential mechanism and effects of pubertal exposure to DEHP on testis development remain unclear.In vivo,postnatal day(PND)21 male rats were gavaged with 0,250,and 500 mg/kg DEHP for ten days.Damage to the seminiferous epithelium and disturbed spermatogenesis were observed after DEHP exposure.Meanwhile,oxidative stress-induced injury and pyroptosis were activated.Both endoplasmic reticulum(ER)stress and mitophagy were involved in this process.Monoethylhexyl phthalate(MEHP)was used as the biometabolite of DEHP in vitro.The GC-1 and GC-2 cell lines were exposed to 0,100μM,200μM,and 400μM MEHP for 24 h.Reactive oxygen species(ROS)generation,oxidative stress damage,ER stress,mitophagy,and pyroptosis were significantly increased after MEHP exposure.The ultrastructure of the ER and mitochondria was destroyed.X-box binding protein 1(XBP1)was observed to be activated and translocated into the nucleus.ROS generation was inhibited by acetylcysteine.The levels of antioxidative stress,ER stress,mitophagy,and pyroptosis were decreased as well.After the administration of the ER stress inhibitor 4-phenyl-butyric acid,both mitophagy and pyroptosis were inhibited.Toyocamycin-induced XBP1 down-regulation decreased the levels of mitophagy and pyroptosis.The equilibrium between pyroptosis and mitophagy was disturbed by XBP1 accumulation.In summary,our findings confirmed that DEHP induced a ROS-mediated imbalance in pyroptosis and mitophagy in immature rat testes via XBP1.Moreover,XBP1 might be the key target in DEHP-related testis dysfunction.

关 键 词：DEHP MITOPHAGY PYROPTOSIS Testicular development XBP1 

分 类 号：R36[医药卫生—病理学]