柚子皮不溶性膳食纤维的提取工艺优化及特性分析  

Optimized Extraction Conditions and Characteristics Analysis of Insoluble Dietary Fiber from Pomelo Peel

作　　者：张文莉[1] 罗银 顾瑶佳 王颖 刘星雨 蔡文博 刘恩岐[1] ZHANG Wenli;LUO Yin;GU Yaojia;WANG Ying;LIU Xingyu;CAI Wenbo;LIU Enqi(Xuzhou University of Technology,School of Food and Biological Engineering,Xuzhou 221018,China)

机构地区：[1]徐州工程学院食品与生物工程学院,江苏徐州221018

出　　处：《食品工业科技》2024年第8期208-215,共8页Science and Technology of Food Industry

基　　金：江苏省自然科学基金青年项目(BK20210078);江苏省高等学校自然科学重大项目(22KJA240003);徐州市科技项目重点研发计划现代农业项目(KC22091)。

摘　　要：为获得复合酶法制备柚子皮不溶性膳食纤维的制备工艺及其吸附特性,实验以柚子皮为原料,采用正交试验优化提取柚子皮不溶性膳食纤维(IDF),并对其特性进行研究。结果表明,料液比1:40 g/mL、淀粉酶的添加量0.6%、淀粉酶的酶解时间90 min、木瓜蛋白酶的添加量0.2%、木瓜蛋白酶的酶解时间30 min为最佳提取条件,该条件下柚子皮IDF得率为55.07%。柚子皮IDF的膨胀力、持水力、持油力和阳离子交换能力分别为2.84±0.09 mL/g、12.54±0.11 g/g、1.54±0.02 g/g和0.36±0.04 mmol/g。此外,体外吸附研究表明,柚子皮IDF对葡萄糖、胆固醇、胆酸钠的吸附能力分别为0.13±0.003 mmol/g、12.27±0.020 mg/g和53.25±0.100 mg/g。FT-IR分析表明,柚子皮IDF具有纤维素类多糖的特征吸收峰,符合膳食纤维的结构。综上,优化工艺后提取的柚子皮IDF具有较好的特性,这为进一步开发利用柚子皮IDF提供了理论依据。In order to obtain the preparation process and adsorption characteristics of insoluble dietary fiber from pomelo peel using composite enzyme method,the orthogonal test was used to optimize the extraction of insoluble dietary fiber from pomelo peel,and its characteristics were studied.The results showed that the optimal extraction conditions were as follows,material-liquid ratio of 1:40 g/mL,amylase addition amount of 0.6%,amylase hydrolysis time of 90 min,papain addition amount of 0.2%and papain hydrolysis time of 30 min.Under these conditions,the yield of pomelo peel IDF was 55.07%.The swelling capacity,water holding capacity,oil holding capacity and cation exchange capacity of pomelo peel IDF were 2.84±0.09 mL/g,12.54±0.11 g/g,1.54±0.02 g/g and 0.36±0.04 mmol/g,respectively.In addition,in vitro adsorption capability test indicated that the adsorption capacity of pomelo peel IDF for glucose,cholesterol and sodium cholate was 0.13±0.003 mmol/g,12.27±0.020 mg/g and 53.25±0.100 mg/g,respectively.FT-IR results showed that pomelo peel IDF had characteristic absorption peaks of cellulose polysaccharides,which was consistent with the structure of dietary fiber.In summary,the extracted pomelo peel IDF exhibited good characteristics after optimizing the process.It can provide theoretical basis for the development and utilization of pomelo peel IDF.

关 键 词：柚子皮 不溶性膳食纤维 工艺优化 理化性质 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]