分级醇沉裙带菜褐藻糖胶及其体外降血糖活性研究  

Study on Gradient Alcohol Precipitation of Fucoidan from Undaria pinnatifida and Its Hypoglycemic Activity in Vitro

作　　者：李灿 吕金博 刘会平[1] 田悦 张声 吕彬斐 薛天睿 张铭冉 LI Can;LÜJinbo;LIU Huiping;TIAN Yue;ZHANG Sheng;LÜBinfei;XUE Tianrui;ZHANG Mingran(College of Food Science and Engineering,Tianjin University of Science&Technology,Tianjin 300457,China)

机构地区：[1]天津科技大学食品科学与工程学院,天津300457

出　　处：《食品工业科技》2024年第8期309-317,共9页Science and Technology of Food Industry

基　　金：国家自然科学基金(31801568);天津市自然科学基金(21YDTPJC00060)。

摘　　要：为研究裙带菜褐藻糖胶分级醇沉组分及其体外降血糖活性,本文以裙带菜为原料,通过CaCl2溶液浸提裙带菜褐藻糖胶后逐级沉淀得到四组多糖样品(UPP-45、UPP-55、UPP-65和UPP-75),分别测定其分子量、总糖含量、糖醛酸含量、蛋白质含量、硫酸根含量及单糖组成,通过紫外光谱扫描、红外光谱以及扫描电镜进行分析,并测定多糖组分对α-淀粉酶和α-葡萄糖苷酶的抑制率来探究其体外降血糖活性。结果表明,醇沉浓度越大,小分子量多糖占比越大。UPP-65的总糖含量最高为84.38%,UPP-45的粗糖蛋白和硫酸根含量与其他组分存在显著性差异(P<0.05);四种多糖组分均为酸性多糖。单糖组成分析可知多糖组分的单糖组成和摩尔百分比存在差异。紫外扫描分析可知多糖组分核酸含量有所不同。红外光谱分析可知四种组分的官能团和糖苷键有不同程度的吸收峰。电镜扫描结果可知,多糖组分微观形态各有不同。多糖组分对α-淀粉酶的抑制无剂量依赖关系,UPP-65的抑制率最高,IC50值为0.008 mg/mL;对α-葡萄糖苷酶的抑制呈量-效关系,UPP-65的IC50值最低,为2.346 mg/mL。综上所述,裙带菜分级醇沉的多糖制备工艺切实可行,四种多糖组分均有一定的体外降血糖活性,且UPP-65效果更好。To study the graded alcohol precipitation components of fucoidan from Undaria pinnatifida and its hypoglycemic activity in vitro,this study used Undaria pinnatifida as raw material,extracted Undaria pinnatifida fucoidan gum through CaCl2 solution,and precipitated it step by step to obtain four sets of polysaccharide samples(UPP-45,UPP-55,UPP-65,and UPP-75).Determined their molecular weight,total sugar content,uronic acid content,protein content,sulfate content and monosaccharide composition and analyzed by ultraviolet spectrum(UV),Fourier transform infrared spectra(FT-IR)and scanning electron microscopy(SEM).The inhibition of polysaccharides onα-amylase andα-glucosidase was determined to explore their hypoglycemic activity in vitro.The results showed that the higher the concentration of alcohol precipitation,the higher the proportion of small molecular weight polysaccharide.The highest total sugar content of UPP-65 was 84.38%,and the protein of the crude polysaccharide and sulfate contents of UPP-45 were significantly different from those of other components(P<0.05).All the four polysaccharide components were acidic polysaccha-rides.The monosaccharide composition analysis showed that the monosaccharide composition and mole percent of polysaccharide components were different.The UV results showed that the content of nucleic acid of polysaccharide components was different.The FT-IR results showed that four components had different levels of absorption peaks of functional groups and glycosidic bonds.The SEM results showed that the microscopic morphology of polysaccharide components was different.The inhibition ofα-amylase was not dose-dependent,and the inhibitory rate of UPP-65 was the highest with IC50 value of 0.008 mg/mL.The inhibition ofα-glucosidase showed a dose-effect relationship,and UPP-65 had the lowest IC50 at 2.346 mg/mL.In summary,the fractional alcohol precipitation of Undaria pinnatifida polysaccharides technology was feasible.All the four polysaccharide components had certain hypoglycemic activit

关 键 词：裙带菜 多糖 分级醇沉 基本组成成分 降血糖活性 

分 类 号：TS201.7[轻工技术与工程—食品科学]