瓦尼桑黄固体发酵产物的化学成分及其生物活性研究  

Chemical constituents and bioactivities of solid fermentedproducts from Sanghuangporus vaninni

作　　者：吴椰 高鹤萍 毛燕妍 黄明慧 徐帮 邹坤 刘呈雄 汪蕾 WU Ye;GAO He-ping;MAO Yan-yan;HUANG Ming-hui;XU Bang;ZOU Kun;LIU Cheng-xiong;WANG Lei(Hubei Key Laboratory of Natural Products Research and Development,Key Laboratory of Functional Yeast(China National Light Industry),College of Biological and Pharmaceutical Sciences,China Three Gorges University;The First College of Clinical Medical Science,China Three Gorges University,Yichang 443002,China)

机构地区：[1]三峡大学生物与制药学院天然产物研究与利用湖北省重点实验室(中国轻工业功能酵母重点实验室) [2]三峡大学第一临床医学院,宜昌443002

出　　处：《天然产物研究与开发》2024年第4期607-615,共9页Natural Product Research and Development

基　　金：天然产物研究与利用湖北省重点实验室(三峡大学)开放基金课题(2022NPRD10)。

摘　　要：对瓦尼桑黄(Sanghuangporus vaninii)固体发酵产物的化学成分及生物活性进行研究。采用超声提取、柱层析以及半制备高效液相色谱等方法分离纯化,通过波谱数据鉴定了化合物的结构,并对所得单体化合物进行体外抗氧化活性、α-葡萄糖苷酶和黄嘌呤氧化酶抑制活性筛选。从瓦尼桑黄固体发酵产物中分离得到17个化合物,分别为(2Z,4E)-γ-ionylideneacetic acid(1)、phellinulin E(2)、phellinulin F(3)、phellinulin G(4)、phellinulin J(5)、phellinulin L(6)、phellinulin M(7)、phellinulin N(8)、elgonenes D(9)、咖啡酸(10)、hispidin(11)、3-hydroxyhispidin(12)、原儿茶酸(13)、4-(3-ethoxy-4-hydroxyphenyl)but-3-en-2-one(14)、丁子香酚(15)、亚油酸甲酯(16)、硬脂酸(17),其中化合物1~9为首次从瓦尼桑黄中分离得到。活性测试结果显示化合物10~13表现出良好的抗氧化活性,对DPPH自由基的半数抑制浓度(IC_(50))分别为16.45、37.52、82.71、50.46μg/mL,对ABTS自由基的IC_(50)值分别为1.43、2.26、34.55、21.60μg/mL,对羟基自由基的IC_(50)值分别为38.43、49.46、86.74、69.16μg/mL。化合物10、11还对黄嘌呤氧化酶表现出良好的抑制作用,IC_(50)值分别为28.73和44.80μg/mL。化合物17具有一定的α-葡萄糖苷酶抑制活性,其IC_(50)值2.31μg/mL。This paper investigated the chemical constituents and their bioactivities of solid fermentation products from Sanghuangporus vaninni.The chemical constituents were isolated by solution extraction,column chromatography and semi-preparative high performance liquid chromatography,and their structures were elucidated by spectral analysis.All these isolated compounds were screened for antioxidant activities and inhibitory effects onα-glucosidase and xanthine oxidase in vitro.A total of 17 compounds were isolated from the extracts of solid fermented products from S.vaninni,and identified as(2 Z,4 E)-γ-ionylideneacetic acid(1),phellinulin E(2),phellinulin F(3),phellinulin G(4),phellinulin J(5),phellinulin L(6),phellinulin M(7),phellinulin N(8),elgonenes D(9),caffeic acid(10),hispidin(11),3-hydroxyhispidin(12),protocatechuic acid(13),4-(3-ethoxy-4-hydroxyphenyl)but-3-en-2-one(14),eugenol(15),methyl linoleate(16)and steraric acid(17).Among them,compounds 1-9 were isolated from S.vaninni for the first time.Compounds 10-13 showed good antioxidant activities in vitro.Their IC_(50) values for DPPH were respectively 16.45,37.52,82.71,50.46μg/mL,for ABTS were 1.43,2.26,34.55,21.60μg/mL and for hydroxyl radicals were 38.43,49.46,86.74,69.16μg/mL.Compounds 10 and 11 also exhibited good inhibitory effects on xanthine oxidase,and their IC_(50) values were 28.73μg/mL and 44.80μg/mL,respectively.Compound 17 demonstrated certain inhibitory effect onα-glucosidase with IC_(50)2.31μg/mL.

关 键 词：瓦尼桑黄 化学成分 体外抗氧化活性 Α-葡萄糖苷酶 黄嘌呤氧化酶 

分 类 号：R284.1[医药卫生—中药学]