模拟胃液结合液质联用研究紫苏对内源性亚硝胺的阻断作用  

Effect of Perilla in Blocking Endogenous Nitrosamines by Simulated Gastric Fluid Combined with Liquid Chromatography-Mass Spectrometry

作　　者：喻凤香[1] 陈煦 陈祖武 曾思景 YU Fengxiang;CHEN Xu;CHEN Zuwu;ZENG Sijing(Hunan Biological Electromechanical Polytechnic,Changsha 410127,China;Hunan Academy of Agricultural Sciences,Changsha 410125,China;Longhui Market Supervision and Management Inspection and Testing Center,Shaoyang 422299,China)

机构地区：[1]湖南生物机电职业技术学院,湖南长沙410127 [2]湖南省农业科学研究院,湖南长沙410125 [3]隆回县市场监督管理检验检测中心,湖南邵阳422299

出　　处：《中国果菜》2024年第4期20-23,共4页China Fruit & Vegetable

基　　金：湖南省自然科学基金项目资助(2023JJ60229)。

摘　　要：为研究蔬菜对亚硝胺的阻断效果,在模拟胃液环境下结合液质联用,研究紫苏提取液对内源性亚硝胺的阻断作用。结果表明,在模拟胃液环境下加入紫苏液与亚硝酸钠,亚硝酸钠清除率可达10.4%。加入紫苏液、亚硝酸钠和二乙胺进行液相色谱质谱检测,空白样品和添加紫苏的样品中二乙基亚硝胺生成量分别为0.2、0.1 ng/mL,抑制率达到50%。添加紫苏的膳食中N-亚硝基二甲基(NDMA)、N-亚硝基二乙基(NDEA)、N-亚硝基二丁基(NDBA)、N-亚硝基吡咯烷(NPYR)含量显著降低。NDMA、NDEA在空白膳食中含量均为0.2 ng/g,紫苏试验组均未检出。NDBA空白对照为0.4 ng/g,紫苏试验组为0.2 ng/g,抑制率为50%;NPYR空白对照为8.6 ng/g,紫苏试验组为4.9 ng/g,抑制率为43%。因此,紫苏对内源性亚硝胺有明显的阻断作用。In order to study the blocking effect of vegetables on nitrosamines,the inhibitory effect of perilla extract on endogenous nitrosamines was studied by combining liquid chromatography-mass spectrometry in a simulated gastric fluid environment.The results indicated that under simulated gastric fluid environment,perilla solution and sodium nitrite were added in simulated gastric juice environment,the sodium nitrite clearance rate could reach 10.4%.Perilla solution,sodium nitrite and diethylamine were added,LC-MS/MS was performed to detect the diethylnitrosamine production of blank sample and perilla added sample at a certain concentration were 0.2 ng/mL and 0.1 ng/mL,and the inhibition rate was 50%.NDMA,NDEA,NDBA and NPYR were significantly decreased in the diet supplemented with perilla.NDMA and NDEA were detected at 0.2 ng/g in the blank diet,but were not detected in the experimental group supplemented with perilla.NDBA blank control was 0.4 ng/g,the experimental group supplemented with perilla was 0.2 ng/g,the inhibition rate was 50%,NPYR blank control was 8.6 ng/g,the experimental group supplemented with perilla was 4.9 ng/g,the inhibition rate was 43%,to provide reference for studying the blocking mechanism of perilla on nitrosamine.Therefore,perilla had a significant blocking effect on endogenous nitrosamines.

关 键 词：亚硝胺 紫苏 模拟胃液 液质联用 亚硝酸钠 

分 类 号：TS201.6[轻工技术与工程—食品科学]