HPLC-MS/MS法测定调味粉中5种罂粟碱含量的不确定度评估  

Uncertainty Evaluation for the Determination of Five Poppy Alkaloids in Seasoning Powder by HPLC-MS/MS Method

作　　者：于晓慧 石梦璇 程凤丽 王晓 YU Xiaohui;SHI Mengxuan;CHENG Fengli;WANG Xiao(Shandong iGiant E-Tech Co.,Ltd.,Jinan 250000;Jinan Foodmate information Technology Co.,Ltd.,Jinan 250000)

机构地区：[1]山东特检科技有限公司,济南250000 [2]济南富美特信息科技有限公司,济南250000

出　　处：《食品工业》2024年第3期270-274,共5页The Food Industry

摘　　要：采用高效液相色谱-串联质谱法测定调味粉中5种罂粟碱含量,对该方法进行不确定度评估。依据BJS201802《食品中吗啡、可待因、罂粟碱、那可丁和蒂巴因的测定》构建不确定度的数学模型,分析整个测定流程,对不确定来源进行分析,计算不确定度分量,获得合成不确定度和扩展不确定度。结果表明:测定调味粉中5种罂粟碱含量过程中不确定度主要来源是标准溶液配制和标准曲线拟合;当调味粉中吗啡、可待因、罂粟碱、那可丁和蒂巴因含量为76.87,76.35,15.77,15.78和15.71μg/kg时,其扩展不确定度分别为8.96,9.72,1.69,2.27和2.49μg/kg (k=2)。试验为调味粉中吗啡、可待因、罂粟碱、那可丁和蒂巴因的检测提供参考和借鉴。The contents of five poppy alkaloids in seasoning powder were determined by high performance liquid chromatography-tandem mass spectrometry,and the uncertainty of the method was evaluated.According to BJS201802 determination of morphine,codeine,papaverine,narcotin and tibaine in food,the mathematical model of morphine and codeine uncertainty was constructed,the whole process of measurement was analyzed,the source of uncertainty was analyzed,and the synthetic uncertainty and extended uncertainty were obtained.The result showed that the main sources of uncertainty in determining the content of five poppy alkaloids in seasoning powder were the preparation of standard solution and the fitting of standard curve;When the contents of morphine codeine,papaverine,narcotin and thebaine in flavoring powder were 76.87,76.35,15.77,15.78 and 15.71 μg/kg,their extended uncertainties were 8.96,9.72,1.69,2.27 and 2.49 μg/kg,respectively(k=2).It could provide reference for the detection of morphine,codeine,papaverine,narcotin and Tibaine in seasoning powder.

关 键 词：高效液相色谱-串联质谱法(HPLC-MS/MS) 调味粉 罂粟碱 不确定度 

分 类 号：TS264[轻工技术与工程—发酵工程] O657.63[轻工技术与工程—食品科学与工程]