大曲中产酯化酶霉菌的分离筛选及发酵条件优化  

Isolation and screening of esterase-producing molds in Daqu and optimization of fermentation conditions

作　　者：曹新志 钟利明 谢进 张凤婷 李书源 杨建刚 CAO Xinzhi;ZHONG Liming;XIE jin;ZHANG Fengting;LI Shuyuan;YANG Jiangang(College of Biological Engineering,Sichuan University of Science and Engineering,Zigong 643001,China;Wuliangye Group Co.,Ltd.,Yibin 644007,China)

机构地区：[1]四川轻化工大学生物工程学院,四川自贡643001 [2]宜宾五粮液股份有限公司,四川宜宾644007

出　　处：《中国酿造》2024年第4期129-134,共6页China Brewing

基　　金：宜宾市五粮液研发项目(CXY2019ZR012)。

摘　　要：为提高大曲己酸乙酯的产量,该研究采用传统培养分离法从五粮液大曲中分离筛选高产酯化酶的霉菌,通过形态学特征观察并结合18S rDNA序列分析鉴定筛选菌株,并以酯化酶活力为响应值,以蔗糖添加量、尿素添加量、发酵温度及发酵时间为自变量,采用单因素试验及响应面试验优化其发酵条件。结果表明,筛选得到一株高产酯化酶的霉菌,编号为GQ342847,经鉴定为总状横梗霉(Lichtheimia ramose),其产酯化酶的最优发酵条件为蔗糖22 g/L,尿素58 g/L,发酵温度35℃,发酵时间6 d,转速150 r/min,在此优化条件下酯化酶活力可达7.67 U/mL,是优化前(5.62 U/mL)的1.3倍。In order to improve the yield of ethyl caproate in Daqu,the high esterase-producing molds from Wuliangye Daqu were isolated and screened by traditional culture separation method,identified and screened by morphological observation combined with 18S rDNA sequence analysis.Using esterase activity as the response value,sucrose addition,urea addition,fermentation temperature and fermentation time as independent variable,the fermentation conditions were optimized by single factor tests and response surface tests.The results showed that a high esterase-producing strain(numbered as GQ342847)was screened and identified as Lichtheimia ramose.The optimal fermentation conditions for esterase production were sucrose 22 g/L,urea 58 g/L,fermentation temperature 35℃,time 6 d,rotate speed 150 r/min.Under this optimal conditions,the esterase activity could reach 7.67 U/ml,which was 1.3 times that of before optimization(5.62 U/ml).

关 键 词：大曲 酯化酶 霉菌 分离 筛选 鉴定 发酵条件优化 

分 类 号：TS261.1[轻工技术与工程—发酵工程]