酿酒酵母发酵白芨产水溶性多糖工艺优化及抗氧化活性研究  

Optimization of fermentation process and antioxidant activity of water-soluble polysaccharides from Bletilla striata by Saccharomyces cerevisiae

作　　者：高超 刘姝[1] 高志贤 张磊[1] 张财顺 GAO Chao;LIU Shu;GAO Zhixian;ZHANG Lei;ZHANG Caishun(College of Petrochemical Engineering,Liaoning Petrochemical University,Fushun 113001,China)

机构地区：[1]辽宁石油化工大学石油化工学院,辽宁抚顺113001

出　　处：《中国酿造》2024年第4期166-172,共7页China Brewing

基　　金：国家自然科学基金(22379059);辽宁省应用基础研究计划项目(2023JH2/101300224);辽宁省教育厅服务地方项目(揭榜挂帅)(LJKFZ20220201);辽宁省教育厅面上项目(LJKMZ20220728);山西省应用基础研究青年基金项目(20210302124338)。

摘　　要：探究耐高温酿酒酵母菌(Saccharomyces cerevisiae)发酵白芨产多糖工艺,以水溶性多糖含量为评价指标,采用单因素试验与响应面试验优化发酵工艺条件,并考察了多糖的抗氧化活性。结果表明,最佳发酵工艺条件为:发酵时间24 h,接种量10%,发酵温度38℃,摇床转速120 r/min,初始pH值5.5,此优化条件下水溶性多糖含量达到了2.80 mg/mL。红外光谱分析表明,提取物具有多糖类物质的特征吸收峰;抗氧化试验结果表明,抗氧化试验表明,发酵组的最大1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除率为66.5%,高于水提对照组,当多糖质量浓度为4 mg/mL时,其是水提对照组DPPH自由基清除率的2.15倍。总之,与水提组相比,酵母菌液体发酵白芨产水溶性多糖具有较好的抗氧化活性。The fermentation process of polysaccharides from Bletilla striata by high temperature resistant Saccharomyces cerevisiae was investigated.Using water-soluble polysaccharides content as the evaluation index,the fermentation process was optimized by single factor experiments and response surface experiments,and the antioxidant capacity of water-soluble polysaccharides were determined.The results showed that the optimal fermentation conditions were as follows:fermentation time 24 h,inoculum 10%,fermentation temperature 38℃,shaker speed 120 r/min,initial pH 5.5.Under the optimal conditions,the water-soluble polysaccharides content reached 2.80 mg/ml.The infrared spectra of the extracts showed that it had the characteristic absorption peak of polysaccharides.The antioxidant tests results showed that the maximum 1,1-diphenyl-2-picrylhydrazyl(DPPH)free radical scavenging rate in the fermentation group was 66.5%,which was higher than that in the water extraction group.When the mass concentration of polysaccharides was 4 mg/ml,the DPPH free radical scavenging rate was 2.15 times that in water extraction control group.In conclusion,compared with the water extraction group,the water-soluble polysaccharides produced by liquid fermentation with S.cerevisiae had stronger antioxidant activity.

关 键 词：耐高温酵母菌 白芨 多糖 发酵工艺 优化 抗氧化活性 

分 类 号：TS261.2[轻工技术与工程—发酵工程]