基于非粮糖蜜的聚羟基脂肪酸酯发酵培养基优化与中试放大  

Optimization of fermentation medium of polyhydroxyalkanoate based on non-grain molasses and pilot scale-up

作　　者：李舒婷 袁凯 李华[1] 周卫强 唐堂 杨小凡 刘海军 李方亮 李凡 李义 彭超[1,2] LI Shuting;YUAN Kai;LI Hua;ZHOU Weiqiang;TANG Tang;YANG Xiaofan;LIU Haijun;LI Fangliang;LI Fan;LI Yi;PENG Chao(College of Food Science and Technology,Henan University of Technology,Zhengzhou 450001,China;Nutrition andHealth Research Institute,COFCO,Beijing 100029,China;COFCO Bioenergy(Yushu)Co.,Ltd.,Changchun 130400,China;Jilin COFCO Biochemical Co.,Ltd.,Changchun 130033,China)

机构地区：[1]河南工业大学粮油食品学院,河南郑州450001 [2]中粮营养健康研究院有限公司,北京100029 [3]中粮生化能源(榆树)有限公司,吉林长春130400 [4]吉林中粮生化有限公司,吉林长春130033

出　　处：《中国酿造》2024年第4期216-223,共8页China Brewing

基　　金：国家重点研发项目(2021YFC2101702);吉林省科技发展计划项目(20220201107GX)。

摘　　要：为促进绿色生物基材料聚羟基脂肪酸酯(PHA)的发展,推进非粮糖蜜发酵等工艺标准化、规模化、绿色化运行,压缩PHA发酵成本,提高生产强度,该研究以盐单胞菌(Halomonos sp.)TD01为发酵菌株,基于非粮糖蜜原料发酵生产PHA。通过单因素试验和响应面法优化确定最佳发酵培养基配方,并调整补料培养基后进行中试放大验证。结果表明,生产PHA最佳发酵培养基配方为糖蜜添加量33.2 g/L,尿素添加量2.5 g/L,硫酸铵添加量2.2 g/L。在此优化条件下,PHA产量为3.12 g/L。通过2 L扩大发酵罐培养得到PHA总产量达到67.36 g/L,较优化前产量增长142.13%;5 kL中试发酵得到PHA总产量为51.16 g/L,较优化前产量增长了83.90%。To promote the development of polyhydroxyalkanoates(PHA),promote the standardization,scale and green operation of non-grain molasses fermentation,reduce PHA fermentation cost and improve production capacity,using Halomonos sp.TD01 as fermentation strain,PHA was produced based on the fermentation of non-grain molasses raw materials.The composition of the fermentation medium was optimized by single factor experiment and response surface method,the optimal fermentation medium formula was determined,and the pilot scale-up test was verified after adjusting the fed medium.The optimum technological formula was obtained as follows:molasses addition 33.2 g/L,urea 2.5 g/L,and ammonium sulfate 2.2 g/L.Under these optimal conditions,PHA was 3.12 g/L.After further optimization and amplification in a 2 L fermenter,the results showed that the PHA total yield reached 67.36 g/L,which was 142.13%higher than that before optimization.The results were verified by 5 kL pilot fermentation experiments,the results showed that the PHA total yield was 51.16 g/L,which was 83.90%higher than that before optimization.

关 键 词：聚羟基脂肪酸酯 糖蜜 发酵培养基 响应面优化 中试放大 

分 类 号：TS249.3[轻工技术与工程—制糖工程] TQ920.1[轻工技术与工程—食品科学与工程]