Lineweaver-Burk双倒数法研究姜酚对α-淀粉酶和α-葡萄糖苷酶的动力学抑制活性  

Lineweaver-Burk Double Reciprocal Method to Study the Kinetic Inhibitory Activity of Gingerol onα-Amylase andα-Glucosidase

作　　者：徐菁 孙浩强 张校志 XU Jing;SUN Haoqiang;ZHANG Xiaozhi(Guiyang University of Information Technology,Guiyang 550025,China;Sports College of Guiyang University,Guiyang 550005,China;Guizhou Business College,Guiyang 550014,China)

机构地区：[1]贵阳信息科技学院,贵州贵阳550025 [2]贵阳学院体育学院,贵州贵阳550005 [3]贵州商学院,贵州贵阳550014

出　　处：《现代食品》2024年第5期201-204,共4页Modern Food

摘　　要：用Lineweaver-Burk双倒数法研究姜酚对α-淀粉酶和α-葡萄糖苷酶的动力学抑制类型。结果表明,姜酚对α-淀粉酶和α-葡萄糖苷酶均有明显的抑制效果,抑制类型分别为竞争型抑制和混合型抑制,其抑制常数K_(ic1)、K_(ic2)分别为3.88和2.67。与α-淀粉酶相比,姜酚与α-葡萄糖苷酶结合的亲和力更好。The Lineweaver-Burk double reciprocal method was used to study the kinetic inhibition type of gingerol onα-amylase andα-glucosidase,and its interaction force was analyzed through thermodynamic parameters.The results showed that gingerol had obvious inhibitory effects onα-amylase andα-glucosidase.The inhibition types were competitive inhibition and mixed inhibition,respectively.The inhibition constants K_(ic1) and K_(ic2) were 3.88 and 2.67,respectively.Compared withα-amylase,the binding affinity of gingerol toα-glucosidase was better.

关 键 词：姜酚 Α-淀粉酶 Α-葡萄糖苷酶 

分 类 号：TS201.4[轻工技术与工程—食品科学]