短期服用蛹虫草对不同状态小鼠的免疫调节作用  

Immunomodulatory Effect of Short-Term Administration of Cordyceps militaris on Mice in Different States

作　　者：张美娜 赵南南 廖思晴 王升厚 王泽[1,3] ZHANG Meina;ZHAO Nannan;LIAO Siqing;WANG Shenghou;WANG Ze(College of Life Science,Shenyang Normal University,Shenyang 110034,China;Liaoning Key Laboratory of Functional Cordyceps militaris,Shenyang 110034,China;Shenyang Functional Cordyceps militaris Industrial Technology Research Institute,Shenyang 110034,China)

机构地区：[1]沈阳师范大学生命科学学院,辽宁沈阳110034 [2]辽宁省功能性蛹虫草重点实验室,辽宁沈阳110034 [3]沈阳市功能性蛹虫草产业技术研究院,辽宁沈阳110034

出　　处：《食品科学》2024年第9期135-143,共9页Food Science

基　　金：辽宁省教育厅服务地方项目(LJKFZ202202662);辽宁省教育厅重点攻关项目(LJKZZ20220116);辽宁省科技厅面上项目(2023-MS-251)。

摘　　要：为研究短期服用蛹虫草对不同状态小鼠的免疫调节作用,探讨其作为免疫调节剂的应用价值,将KM系小鼠随机分为低倍组(DB组)、高倍组(GB组)和对照组(CK组),DB组和GB组连续灌胃不同剂量蛹虫草水溶液21 d,CK组灌胃同等剂量蒸馏水。测定小鼠体质量、脏器指数、血清免疫球蛋白(immunoglobulin,Ig)G、IgM、白细胞介素(interleukin,IL)-2和IL-10含量,外周血CD3^(+)CD4^(+)和CD3^(+)CD8^(+)T淋巴细胞亚群含量,肠道菌群组成结构及空肠Toll样受体(Toll-like receptor,TLR)2、TLR4、髓样分化因子88及核因子(nuclear factor)-κB表达量的变化。腹腔注射80 mg/kg mb环磷酰胺溶液诱导小鼠免疫抑制状态,在蛹虫草水溶液灌胃的第0、7、14、21天取材检测。结果显示,不同剂量蛹虫草对小鼠体质量、脏器指数、血清IgG、IgM、IL-2和IL-10水平、外周血CD3^(+)CD4^(+)和CD3^(+)CD8^(+)T淋巴细胞亚群含量均未产生显著影响(P>0.05)。蛹虫草可以上调小鼠肠道菌群Shannon指数和操作分类单元数量,增加肠道菌群多样性和丰富度。肠道益生菌Lactobacillus和Alistipes相对丰度明显升高(P<0.05),炎症促生菌Clostridium XIVa、Helicobacter及Lachnospiracea_incertae_sedis相对丰度显著降低(P<0.05)。不同剂量蛹虫草均引起小鼠空肠组织TLR4表达量显著降低(P<0.05),NF-κB表达量的降低程度与蛹虫草灌胃剂量呈正相关。蛹虫草能够提前恢复环磷酰胺导致的脏器损伤,显著提高免疫抑制小鼠IgM、IgG和IL-2水平(P<0.05),促进外周血CD3^(+)CD4^(+)和CD3^(+)CD8^(+)T淋巴细胞亚群的增殖。综上,短期服用蛹虫草可以降低肠道炎症的发生风险,不会引起正常免疫状态的失衡;蛹虫草可以通过增强体液免疫和细胞免疫促进机体免疫抑制状态的恢复,可用于免疫调节剂的开发和利用。In order to investigate the immunomodulatory effects of short-term administration of Cordyceps militaris on mice in different states and explore its application value as an immunomodulator,Kunming(KM)mice were randomly divided into a low-dose group(DB),a high-dose group(GB)and a control check group(CK).The mice in the DB and GB groups were continuously gavaged with different doses ofan aqueous suspension of C.militaris powder for 21 days,while the CK group was given the same dose of distilled water.Changes in body mass,organ indexes,serum immunoglobulin G(IgG),IgM,interleukin(IL)-2 and IL-10 levels,the percentages of CD3^(+)CD4^(+)and CD3^(+)CD8^(+)T lymphocyte subsets in peripheral blood,intestinal flora composition and Toll-like receptor(TLR)2,TLR4,myeloid differentiation factor(MyD)88 and nuclear factor(NF)-κB expression in the jejunum were measured.Immunosuppressed mice induced by intraperitoneal injection of 80 mg/kg mb cyclophosphamide solution were intragastrically administered with C.militaris for 21 consecutive days.Immune indexes were detected after 0,7,14 and 21 days of administration.The results showed that administration of C.militaris at each dose did not cause significant changes in body mass,organ indexes,serum IgG,IgM,IL-2 and IL 10 levels or the percentages of CD3^(+)CD4^(+)and CD3^(+)CD8^(+)T lymphocyte subsets in the peripheral blood of mice(P>0.05).C.militaris could up-regulate the Shannon index and the number of operational taxonomic units(OTU)in the intestinal flora of mice,and increase the diversity and richness of the intestinal flora.The relative abundances of the probiotics Lactobacillus and Alistipes were significantly increased(P<0.05).The relative abundances of the inflammation-promoting bacteria Clostridium XIVa,Helicobacter and Lachnospiracea_incertae_sedis were significantly decreased(P<0.05).The expression of TLR4 in jejunum tissues of mice was significantly decreased by all doses of C.militaris(P<0.05),and the reduction of NF-κB expression was positively correlated with the do

关 键 词：蛹虫草 免疫调节 肠道菌群 免疫抑制 

分 类 号：TS201.4[轻工技术与工程—食品科学]