林麝肠道中乳酸菌的分离筛选及益生特性  

Isolation and Screening of Lactic Acid Bacteria from the Gut of Forest Musk Deer for Probiotic Properties

作　　者：訾静[1,2] 王琰 李亮亮[3] 张琨 万一 ZI Jing;WANG Yan;LI Liangliang;ZHANG Kun;WAN Yi(Shaanxi Institute of Microbiology,Xi’an 710043,China;Engineering Center of Qinling Mountains Natural Products,Shaanxi Academy of Sciences,Xi’an 710043,China;College of Science,Xi’an University of Technology,Xi’an 710054,China)

机构地区：[1]陕西省微生物研究所,陕西西安710043 [2]陕西省科学院,秦岭天然产物工程中心,陕西西安710043 [3]西安理工大学理学院,陕西西安710054

出　　处：《食品科学》2024年第8期79-86,共8页Food Science

基　　金：“秦药”研发重点实验室(2021-QYPT-001);“秦药”质量提升工程项目(2021-QYZL-01);秦巴山区微生物资源发掘与利用创新团队(2020TD-050)。

摘　　要：本研究旨在从健康林麝粪便中分离筛选用于改善林麝健康状况的潜在益生菌株,并对其进行体外益生性评价。共分离获得12株乳酸菌,结合细胞个体形态观察及细菌16S rDNA序列鉴定,体外评价其耐酸和耐胆盐能力,测定其对5种目标病原菌(大肠埃希氏菌Escherichia coli ATCC 25922、金黄色葡萄球菌Staphylococcus aureus ATCC 25923、铜绿假单胞菌Pseudomonas aeruginosaPAO1、肠炎沙门氏菌Salmonella enteritidisATCC 13076和化脓隐秘杆菌Arcanobacterium pyogenes LSN3)的拮抗活性、自聚集和疏水性、抗生素敏感性、抗药性基因型和生长曲线。结果显示,5株植物乳植杆菌(Lactiplantibacillus plantarum)和1株乳酸片球菌(Pediococcus acidilactici)在pH 4酸性条件或质量分数0.5%胆盐环境中,存活率分别达88.5%和87%以上;能够抑制前4种目标病原菌(抑菌圈直径17.20~20.19 mm)和化脓隐秘杆菌LSN3(抑菌圈直径14.23~15.53 mm);与对照菌株副干酪乳酪杆菌(L. paracasei)L22具有相近的自聚集(53.1%~56.2%)和表面疏水率(52.9%~56.0%);对链霉素、环丙沙星和万古霉素耐药;携带万古霉素抗性基因vanX;具有快速生长的特点(培养24 h后OD600分别达9.0和7.1左右)。本研究筛选出的6株乳酸菌可作为后续开发林麝用益生菌制剂的一种可靠的菌株来源。This study aimed to isolate and select potential probiotic strains from the feces of healthy forest musk deer and to evaluate their in vitro probiotic characteristics.Twelve isolates were obtained and identified by cell morphology and bacterial 16S rDNA sequencing.Their survival under low pH and bile salt conditions,antagonistic activities against five pathogens(Escherichia coli ATCC 25922,Staphylococcus aureus ATCC 25923,Pseudomonas aeruginosa PAO1,Salmonella enteritidis ATCC 13076 and Arcanobacterium pyogenes LSN3),auto-aggregation characteristics,hydrophobicity,antibiotic susceptibility and antibiotic resistance genotypes,and growth curves were evaluated.The results showed that five Lactiplantibacillus plantarum isolates and one Pediococcus acidilactici isolate survived at pH 4,or in 0.5%bile salt with survival rates of more than 88.5%and 87%,respectively.All of them inhibited the five pathogens with diameters of inhibition zones of 17.20-20.19 mm for E.coli ATCC 25922,S.aureus ATCC 25923,P.aeruginosa PAO1 and S.enteritidis ATCC 13076 and of 14.23-15.53 mm for A.pyogenes LSN3.They had similar auto-aggregation capacity(53.1%–56.2%)and surface hydrophobicity(52.9%–56.0%)to L.paracasei L22.They were resistant to streptomycin,ciprofloxacin and vancomycin,carried the vanX gene and rapidly grew;the optical density at 600 nm(OD600)reached 9.0 and 7.1 for L.plantarum and P.acidilactici from the gut of forest musk deer after 24 h incubation,respectively.Our findings demonstrated that these six isolates can be used as sources for the development of probiotic preparations intended for forest musk deer.

关 键 词：林麝 乳酸菌 分离 鉴定 益生性 

分 类 号：TS201.3[轻工技术与工程—食品科学]