Prostaglandin D2 regulation of autophagy affects stemness of gastric cancer stem cells  

作　　者：TIAN Heng-jin WANG Fei-fan GAO Peiyao CHEN Amin WANG Na ZHANG Qiang 

机构地区：[1]Department of Clinical Laboratory,the First Affiliated Hospital of Bengbu Medical University,Bengbu 233004,China [2]Department of Blood Transfusion,the First Affiliated Hospital of Naval Medical University,Bengbu 233004,China [3]Key Laboratory of Cancer Research and Clinical Laboratory Diagnosis,Bengbu Medical University,Bengbu 233004,China

出　　处：《Journal of Hainan Medical University》2024年第4期15-21,共7页海南医学院学报（英文版）

基　　金：Natural Science Foundation of Anhui Province(No.1908085MH258);Scientific Research and Innovation Project of Bengbu Medical College(No.Byycxz21004)。

摘　　要：Objective:To explore the effect and mechanism of prostaglandins D2(PGD2)on the stemness of gastric cancer stem cells(GCSCs).Methods:7901-GCSCs were enriched by serum-free culture method;then the positivity rate of CD44,a stemness marker,was detected by flow cytometry in serum-free cultured 7901-GCSCs;the sphere-forming ability was detected by the sphere-forming assay after stimulation with different concentrations of PGD2(2.5,5,10)μg/mL,and the expression of stemness-related indicators(OCT4,CD44)and autophagyrelated proteins(LC3,Beclin-1)after PGD2 stimulation was detected by the western blot assay in different concentrations.The expression of stemness-related indexes(OCT4,CD44)and autophagy-related proteins(LC3,Beclin-1)were detected by Western blot assay after stimulation with different concentrations of PGD2.The expression of autophagy-related proteins after stimulation with different concentrations of CQ(2.5,5,10)μM was detected by Western blot experiment.The protein expression of autophagy-related proteins(LC3,Beclin-1)and stemness-related indexes(OCT4,CD44)was detected by Western blot experiment after PGD2 as well as PGD2+CQ treatment.Results:Flow cytometry results showed that the expression of CD44 positivity was increased in serum-free cultured 7901-GCSCs compared with gastric cancer cells SGC-7901(P<0.05),which fulfilled the needs of subsequent experiments.The results of stem cell spheroid formation assay showed that the spheroid formation ability of 7901-GCSCs in the PGD2 group was significantly weakened compared with that of the DMSO group(P<0.05).Western blot results showed that the protein expression of stemness-related indexes(OCT4,CD44)was down-regulated in the 7901-GCSCs in the PGD2 group compared with that of the DMSO group(P<0.05),and the expression of autophagy-related proteins(LC3,Beclin-1)expression increased(P<0.05).Compared with the DMSO group,the expression of autophagy-related proteins(LC3,Beclin-1)was decreased in the CQ group(P<0.05).Western blot results also showed that the expressio

关 键 词：PGD2 Gastric cancer Gastric cancer stem cells AUTOPHAGY STEMNESS 

分 类 号：R730.2[医药卫生—肿瘤]