自身荧光多肽探针快速高通量检测铜离子  

Rapid and high-throughput detection of copper ions using self fluorescent polypeptide probe

作　　者：谢紫微 许迪雅 张新 王芳 穆岚 张琳[1] XIE Zi-wei;XU Di-ya;ZHANG Xin;WANG Fang;MU Lan;ZHANG Lin(College of Food Science and Engineering,Central South University of Forestry and Technology,Changsha 410004,Hunan,China;Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning,Changsha 410117,Hunan,China;Hunan Institute of Commodity Quality Inspection,Changsha 410117,Hunan,China)

机构地区：[1]中南林业科技大学食品科学与工程学院,湖南长沙410004 [2]食品安全监测与预警湖南省重点实验室,湖南长沙410117 [3]湖南省产商品质量检验研究院,湖南长沙410117

出　　处：《粮食与油脂》2024年第5期147-152,共6页Cereals & Oils

基　　金：食品安全监测与预警湖南省重点实验室开放基金项目(2020KFJJ03);湖南省研究生科研创新项目(QL20220182);2023年中南林业科技大学“克明食品”研究生科技创新项目(2023KMCX12,2023KMCX02);国家级大学生创新创业训练计划项目(S202310538020)。

摘　　要：构建了以组氨酸(H)-色氨酸(W)-组氨酸(H)-组氨酸(H)-谷氨酸(E)为氨基酸序列的荧光多肽探针HWHHE,结合酶标仪,可实现对水溶液中Cu^(2+)的快速高通量检测。结果表明:在优化的检测条件下(25℃、pH 7.4),探针多肽荧光HWHHE强度与Cu^(2+)浓度(0~1.0μmol/L)呈线性关系(R^(2)=0.997),检出限为2.67μg/L,远低于国家标准规定的限量,且加标回收率为93.7%~102.1%。采用高通量法检测实际样品(自来水以及饮用水)中Cu^(2+)浓度,其结果与用分光光度法测定结果无显著差异(P>0.05),证明荧光多肽探针HWHHE可结合酶标仪实现水溶液中Cu^(2+)的高通量快速检测。A fluorescent polypeptide probe HWHHE was constructed with an amino acid sequence of histidine(H)-tryptophan(W)-histidine(H)-histidine(H)-glutamic acid(E).Combined with microplate reader,it could achieve rapid and high-throughput detection of Cu^(2+)in aqueous solutions.The results showed that under optimized detection conditions(25℃,pH 7.4),the fluorescence intensity of HWHHE probe had a linear relationship with the Cu^(2+)concentration(0-1.0μmol/L)(R^(2)=0.997).The limit of detection was 2.67μg/L,which was lower than the allowable limit of national standard,and the recovery rate of the HWHHE fluorescence polypeptide probe for the determination of Cu^(2+)was 93.7%-102.1%.The high-throughput method was used to detect the content of Cu^(2+)in actual samples(tap water and drinking water),and the results had no significant difference with the results detected with spectrophotometric measurement(P>0.05),which suggested that the HWHHE fluorescent polypeptide probe could be combined with a microplate reader to achieve rapid and high-throughput detection of Cu^(2+)in aqueous solutions.

关 键 词：多肽荧光探针 选择性 铜离子 水 高通量检测 

分 类 号：TS207.51[轻工技术与工程—食品科学]