膜分离法提高麦芽四糖糖浆纯度的研究  

Study on improving the purity of maltotetraose syrup by membrane separation

作　　者：李佳明 张宏建[1,2] 王靓[1,2] 陈旭升[1,2] 张建华[1,2] LI Jiaming;ZHANG Hongjian;WANG Liang;CHEN Xusheng;ZHANG Jianhua(School of Biotechnology,Jiangnan University,Wuxi 214122,China;Key Laboratory of Industrial Biotechnology,Ministry of Education(Jiangnan University),Wuxi 214122,China)

机构地区：[1]江南大学生物工程学院,江苏无锡214122 [2]江南大学工业生物技术教育部重点实验室,江苏无锡214122

出　　处：《食品与发酵工业》2024年第9期182-187,195,共7页Food and Fermentation Industries

摘　　要：该研究拟采用膜分离法将麦芽四糖糖浆中麦芽四糖的纯度提高到80%以上。研究结果表明,麦芽四糖糖浆经截留分子质量为1000 Da的膜超滤后,大部分糊精被去除,但小分子糖含量几乎没有损失;以渗透通量、各糖截留率、分离系数为评价指标,筛选出最适合用于提高麦芽四糖纯度的纳滤膜的截留分子质量为150~300 Da;实验确定的纳滤膜最优工艺参数为跨膜压差1.0 MPa,操作温度35℃,进料质量浓度15 g/L,纳滤后麦芽四糖纯度由73.40%提升至78.20%;进一步采用浓缩因子为2的五阶段间歇渗滤,截留液中麦芽四糖的纯度达到87.14%。膜分离工艺不能完成相近分子质量低聚糖的绝对分离,但利用各糖截留率的差异,可以提高产品中麦芽四糖的纯度。This study aimed to investigate improving the purity of maltotetraose(G4)to over 80%from its syrup by the ultrafiltration(UF)-nanofiltration(NF)membranes technology.Results showed that high recovery of total sugar and high rejection of dextrin could be obtained in permeate generated by the UF membrane XT(cut-off of 1000 Da).The NF membrane NFX(cut-off of 150-300 Da)was most suitable for improving the purity of G4 by using permeation flux,rejection rate,and separation coefficient as evaluation indexes.The optimal nanofiltration membrane process parameters were that transmembrane pressure was 1.0 MPa,the operating temperature was 35℃,the total sugar concentration was 15 g/L,and the purity of G4 after nanofiltration was increased from 73.40%to 78.20%.Further,a five-stage intermittent infiltration with a concentration factor of 2 was used,and the purity of G4 in the retentate reached 87.14%.The membrane separation process could not complete the absolute separation of oligosaccharides with similar molecular weight,but the purity of G4 in the product could be increased by using the different retention rates of each sugar,which would be of great significance for further study on the application value of G4.

关 键 词：麦芽四糖 超滤 纳滤 间歇渗滤 糊精分子质量分布 

分 类 号：TS244[轻工技术与工程—制糖工程]