大麦中抑制镰刀霉菌的乳酸菌筛选及其在制麦中的应用  

Screening of Lactiplantibacillus plantarum inhibiting Fusarium graminearumin barley and its application in malting

作　　者：王羽 孙笑天 富洋 俞志敏[1] 杨帆[1] 佟晔 WANG Yu;SUN Xiaotian;FU Yang;YU Zhimin;YANG Fan;TONG Ye(School of Biological Engineering,Dalian Polytechnic University,Dalian 116034,China;Dalian Modern Agricultural Production Development Service Center,Dalian 116081,China)

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034 [2]大连市现代农业生产发展服务中心,辽宁大连116081

出　　处：《大连工业大学学报》2024年第2期96-101,共6页Journal of Dalian Polytechnic University

基　　金：国家自然科学基金项目(31671796);辽宁省教育厅高等学校基本科研项目(LJKQZ2021114);大连市重点研发计划(2022YF16SN064)。

摘　　要：大麦表面存在多种微生物,其中镰刀霉菌及其分泌的真菌毒素会对制麦过程及麦芽品质产生影响。为了消除镰刀霉菌带来的不利影响,利用MRS选择性培养基从大麦表面分离筛选出一株对镰刀霉菌有明显抑制作用的菌株。根据菌株的形态学观察、生理生化试验以及16S rDNA基因序列分析鉴定,确定该菌株为植物乳杆菌(Lactiplantibacillus plantarum),命名为BY66。在制麦过程中,添加0.5 mL/kg的BY66发酵液能使禾谷镰刀霉的生物量和呕吐毒素含量分别降低56.2%和79.1%。BY66发酵液能消除禾谷镰刀霉菌在大麦发芽和麦芽质量方面产生的负面影响,与侵染大麦相比,添加0.5 mL/kg BY66发酵液后的大麦发芽率提高了31%。There are various microorganisms on the surface of barley, among which Fusarium graminearum and its secreted mycotoxins can affect the malting process and malt quality. To eliminate the adverse effects of F. graminearum , a strain with significant inhibitory effect was isolated and screened from the surface of barley using MRS selective culture medium. Based on morphological observation, physiological and biochemical experiments, and 16S rDNA gene sequence analysis, the strain was identified as Lactiplantibacillus plantarum named BY66. During the wheat making process, the biomass and deoxynivalenol content of \%F. graminearum\% could be reduced by 56.2% and 79.1%, respectively, through adding 0.5 mL/kg of BY66 fermentation broth. The negative effects of \%F. graminearum\% on barley germination and malt quality also could be eliminated by adding BY66 fermentation broth. Compared with the infected barley, the germination rate of barley could be increased by 31% with the addition of 0.5 mL/kg BY66 fermentation broth.

关 键 词：制麦 植物乳杆菌 禾谷镰刀霉菌 真菌毒素 

分 类 号：Q939.95[生物学—微生物学]