定向驯化罗伊氏粘液乳杆菌F22A089尿酸降解能力研究  

Studies on the Targeted Domestication of Limosilactobacillus reuteri F22A089for Uric Acid Degradation Ability

作　　者：刘欣蕾 甄冬玉 张家超[1] Liu Xinlei;Zhen Dongyu;Zhang Jiachao(School of Food Science and Engineering,Hainan University,Haikou 570100)

机构地区：[1]海南大学食品科学与工程学院,海口570100

出　　处：《中国食品学报》2024年第4期9-21,共13页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金优秀青年基金项目(32222066)。

摘　　要：以罗伊氏粘液乳杆菌F22A089对核苷降解能力为例,探究增强其核苷降解能力的驯化策略,为获得高降尿酸能力菌株及对益生菌特定功能的定向驯化提供理论依据。采用体外驯化和体内驯化两种方法对益生菌降解核苷的能力进行驯化。结果发现,体外驯化无法快速增强益生菌株的核苷降解能力,驯化3周后,菌株降解核苷的能力仍无显著变化。而通过饮食驱动小鼠体内的高尿酸肠道环境对益生菌株进行体内驯化的研究表明,罗伊氏粘液乳杆菌F22A089驯化株增强了对核苷的降解能力,部分菌株对鸟苷的降解率达到原始菌株的2倍,说明通过宿主肠道选择压力对益生菌株定向驯化具有可行性。对驯化菌株进行全基因组及转录组测序及突变位点分析,共注释到3个与降尿酸能力相关的突变基因(pbuG_1,rihB和yusV),这些功能基因的突变与驯化菌株降尿酸能力增强密切相关。本研究率先通过体内驯化的方式定向驯化菌株并提高其对尿酸的降解能力,为通过宿主肠道定向驯化益生菌,增强其益生功效,提供了切实可行的驯化方法。This study took Limosilactobacillus reuteri F22A089 as an example to explore domestication strategies to enhance its nucleoside degradation ability,which provided theoretical basis for obtaining strains with high uric acid lowering ability and targeted domestication of specific functions of probiotics.Two methods of in vitro and in vivo domestication were used to domesticate the ability of probiotics to degrade nucleosides.The results showed that the in vitro domestication method could not rapidly improve the nucleoside degradation ability of probiotics strains,and the nucleoside degradation ability of probiotics strains remained unchanged after 3 weeks of domestication.The study on in vivo domestication of probiotic strains by high-uric acid intestinal environment in mice driven by diet found that the domestication of Limosilactobacillus reuteri F22A089 increased the degradation ability of nucleoside,and the degradation rate of guanosine in some strains reached twice that of the original strain,indicating that it is feasible to target domestication of probiotic strains by host intestinal selection pressure.Whole genome and transcriptome sequencing and mutation site analysis were performed in domesticated strains.Three mutant genes(pbuG_1,rihB and yusV)related to uric acid lowering ability were noted.The mutations of these functional genes were closely related to the enhancement of uric acid lowering ability in domesticated strains.This study was the first to domesticate strains in vivo and improve their ability to degrade uric acid,providing a feasible domestication method for domesticating probiotics through the host gut and enhancing their probiotic efficacy.

关 键 词：益生菌 定向驯化 体内驯化 降尿酸 罗伊氏粘液乳杆菌F22A089 

分 类 号：TS201.3[轻工技术与工程—食品科学]