黄嘌呤氧化酶抑制剂微胶囊的制备  

Preparation of Microcapsule Preparation of Xanthine Oxidase Inhibitor

作　　者：崔方超 李兰玲 韩瑜娟 王当丰 檀茜倩 吕欣然 励建荣 李婷婷 Cui Fangchao;Li Lanling;Han Yujuan;Wang Dangfeng;Tan Xiqian;LüXinran;Li Jianrong;Li Tingting(College of Food Science and Engineering,Bohai University,National&Local Joint Engineering Research Center of Storage,Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products,Jinzhou 121013,Liaoning;College of Life Science,Dalian Minzu University,Dalian 116600,Liaoning)

机构地区：[1]渤海大学食品科学与工程学院生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,辽宁锦州121013 [2]大连民族大学生命科学学院,辽宁大连116600

出　　处：《中国食品学报》2024年第4期223-234,共12页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金重点项目(U20A2067);辽宁省自然科学基金博士科研启动基金计划项目(2022-BS-301)。

摘　　要：目的:为提高黄嘌呤氧化酶(XOD)抑制剂稳定性和生物可及性,采用喷雾干燥法制备XOD抑制剂的微胶囊。方法:以接枝度为指标,单因素实验优化糖基化反应产物的制备工艺,通过傅里叶红外光谱、紫外光谱、荧光光谱等对糖基化产物进行结构表征,探究不同反应时间对功能性质的影响。以大豆分离蛋白(SPI)和普鲁兰多糖的糖基化反应产物为壁材,包埋XOD抑制剂,利用SEM、XRD衍射、差示扫描量热分析等对微胶囊进行结构表征。研究微胶囊在模拟消化液中的释放率及贮藏稳定性。结果:糖基化产物最优制备工艺:蛋白含量1.5%,蛋白和糖的质量比1∶2,反应时间20 min,接枝度最高达35%以上。表征结果表明SPI糖基化改性成功,而且溶解度提高40%,乳化活性和乳化稳定性分别提高2倍和4倍,表面疏水性指数降低225,有利于作为微胶囊壁材。包埋对羟基肉桂酸(HCA)和橙皮素(HES)后,通过SEM观察到微胶囊大小均匀、表面光滑。XRD衍射光谱和差示扫描量热分析验证了HCA和HES被成功包埋。微胶囊使抑制剂的生物利用率提高了50%,且微胶囊的贮藏稳定性较好。结论:本试验成功研发了基于乳酸菌代谢物的XOD抑制剂微胶囊制剂。高效包埋的XOD抑制剂微胶囊为HCA和HES作为生物活性物质用于功能性食品的开发和利用提供了依据。Objective:To improve the stability and bioavailability of xanthine oxidase(XOD)inhibitors,microcapsules based on XOD inhibitors were prepared by spray drying method.Methods:Using the grafting degree as an index,the preparation process of the glycosylation reaction product was optimized by single factor,and the structure was characterized by Fourier transform infrared spectroscopy,ultraviolet spectroscopy,fluorescence spectroscopy,etc.,to explore the effect of different reaction times on the functional properties.The microcapsules were characterized by SEM,XRD and differential scanning calorimetry,using the glycosylation reaction product of soybean protein isolate(SPI)and pullulan as the wall material.The release rate and storage stability of microcapsules in simulated digestive juice were studied.Results:The optimal preparation process of glycosylation products was that the protein content was 1.5%,the mass ratio of protein and sugar was 1∶2,the reaction time was 20 min,and the grafting degree was up to more than 35%.The characterization results showed that the glycosylation modification of SPI was successful,and the solubility was increased by 40%,the emulsifying activity and emulsifying stability were increased by 2 times and 4 times,respectively,and the surface hydrophobicity index was reduced by 225,which was beneficial to be used as a microcapsule wall material.After embedding p-hydroxycinnamic acid(HCA)and hesperetin(HES),SEM,observed microcapsules with uniform size and smooth surface,XRD diffraction spectrum and differential scanning calorimetry verified the successful embedding of HCA and HES.Microcapsules increased the bioavailability of inhibitors by 50%,and the storage stability of microcapsules was good.Conclusion:This experiment successfully developed the XOD inhibitor microcapsule formulation based on lactic acid bacteria metabolites.The high-efficiency embedded XOD inhibitor microcapsules provide the basis for the development and utilization of HCA and HES as biologically active substances in fun

关 键 词：黄嘌呤氧化酶 高尿酸血症 抑制剂 微胶囊 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]