尿素包合法富集裂壶藻油中多不饱和脂肪酸的工艺  

Enrichment of Polyunsaturated Fatty Acids in Oil from Schizochytrium sp.by Urea Inclusion Method

作　　者：孙兆敏 龚菲菲 刘萌 李颖 宋金平 黄海华 倪辉 余蕾[1] SUN Zhaomin;GONG Feifei;LIU Meng;LI Ying;SONG Jinping;HUANG Haihua;NI Hui;YU Lei(College of Marine Biology,Xiamen Ocean Vocational College,Xiamen,Fujian 361102,China;Weihai Key Laboratory of Marine Functional Lipids,Weihai,Shandong 266003,China;Weihai Boow Foods Co.,Ltd.,Weihai,Shandong 266003,China;Fujian High Fortune Bio-Tech Corporation,Fuzhou,Fujian 350311,China)

机构地区：[1]厦门海洋职业技术学院海洋生物学院,福建厦门361102 [2]威海市海洋功能性脂质重点实验室,山东威海266003 [3]威海博宇食品有限公司,山东威海266003 [4]福建海富特生物科技股份有限公司,福建福州350311

出　　处：《福建技术师范学院学报》2024年第2期79-87,共9页JOURNAL OF FUJIAN POLYTECHNIC NORMAL UNIVERSITY

基　　金：福建省中青年教师教育科研项目(JAT210803、JAT210801);厦门海洋职业技术学院科研项目(高层次人才科研专项,KYG202207、KYG202108);厦门海洋职业技术学院教师教学创新团队项目(食品智能加工技术专业).

摘　　要：将冬化裂壶藻油转化为乙酯型藻油,以此为原料研究了尿包合法富集多不饱和脂肪酸的工艺.通过单因素试验比较不同的后处理方式、冷却结晶温度、时间、乙醇浓度、脲酯比,以及醇脲比对尿素包合的影响,并通过正交试验获得较优的工艺条件.结果表明,采用优选的后处理方式可提高参与包合作用的尿素比例,从而使非尿素包合组分中含有更高的多不饱和脂肪酸,在较优的工艺条件下,乙酯型藻油的二十二碳六烯酸和二十二碳五烯酸含量可由47.17%、13.93%分别提高到73.20%、19.87%.This paper focused on the concentration of polyunsaturated fatty acids(PUFA)in ethyl esters(EE)converted from winterized Schizochytrium sp.oil via urea inclusion method.The impact of various post-treatment methods,cooling temperatures,cooling times,ethanol concentrations,urea/EE ratios,and ethanol/urea ratios,on the urea inclusion process was investigated through one-way tests,and orthogonal tests were conducted to determine the optimal process conditions.The results demonstrated that the utilization of the optimized post-treatment method significantly enhanced the involvement of urea in the inclusion process with EE,resulting in an increased PUFA content in the non-urea included fractions.Under the optimized conditions,the contents of docosahexaenoic acid and docosapentaenoic acid in the EE-type microalgal oil were improved from 47.17%and 13.93%to 73.20%and 19.87%,respectively.

关 键 词：尿素包合 裂壶藻 二十二碳六烯酸(DHA) 二十二碳五烯酸(DPA) 多不饱和脂肪酸 

分 类 号：TS254.5[轻工技术与工程—水产品加工及贮藏工程] Q814.9[轻工技术与工程—食品科学与工程]