高光纯D-乳酸生产菌株假肠膜明串珠菌HL64-1的分离鉴定及其发酵特性  

Isolation,Identification and Fermentation Characterization of Leuconostoc pseudomesenteroides HL64-1 Producing D-Lactic Acid with High Optical Purity

作　　者：黄筱萍[1] 王通 李鹏 刘兰[1] HUANG Xiaoping;WANG Tong;LI Peng;LIU Lan(Institution of Microbiology,Jiangxi Academy of Sciences,Nanchang 330096,China)

机构地区：[1]江西省科学院微生物研究所,江西南昌330096

出　　处：《食品工业科技》2024年第11期133-141,共9页Science and Technology of Food Industry

基　　金：江西省科学院重点研发计划项目(2021YSBG21014,2022YSBG21007,2022YSBG22011)。

摘　　要：从自然界中筛选分离产酸菌是获得高光学纯度乳酸生产菌株有效的途径之一。从腐烂果实中分离获得一株产高光学纯度D-乳酸菌株HL64-1,经形态学、16S rDNA序列分析、序列相似性Blast比对分析鉴定为假肠膜明串珠菌(Leuconostoc pseudomesenteroides)。在基础发酵培养基中摇瓶发酵24 h,产D-乳酸的量达到62.18 g/L,产酸速率达2.59 g/(L·h),光学纯度达99.90%(ee);在5 L发酵罐中放大培养,通过补加碳源,发酵72 h,D-乳酸产量达到78.74 g/L,平均产酸速率达1.09 g/(L·h)。该菌株可以有效利用农业副产物花生饼粉和棉籽粉作为替代氮源以降低发酵原料成本。该菌还可利用木糖产生D-乳酸,且葡萄糖能显著提高木糖的利用效率,极具工业应用前景。Isolation and identification of acid-producing bacteria from environmental samples is still one of important and effective approaches to obtain strains producing lactic acid with high optical purity.In this study,an isolate strain named HL64-1,producing D-lactic acid with high optical purity,was isolated from rotten fruit.And HL64-1 was characterized as Leuconostoc pseudomesenteroides based on morphology,16S rDNA sequence analysis and sequence similarity Blast analysis.After fermentation for 24 h,62.18 g/L D-lactic acid was produced with an optical purity of 99.90%(ee)in basic fermentation culture.The acid production rate was 2.59 g/(L·h).Scale-up fermentation of L.pseudomesenteroides HL64-1 in 5-L fermenter for 72 h produced 78.74 g/L D-lactic acid by replenishment of carbon sources,and the average acid production rate was 1.09 g/(L·h).Moreover,L.pseudomesenteroides HL64-1 was found to be capable of using effectively cheap raw materials,such as agricultural by-products peanut cake meal and cottonseed meal,as alternative nitrogen sources,leading a reduction of fermentation cost.In addition,L.pseudomesenteroides HL64-1 could metabolize xylose into D-lactic acid.Co-fermentation of glucose and xylose mixtures showed that glucose significantly enhanced the utilization efficiency of xylose,making L.pseudomesenteroides HL64-1 to be a promising strain for pure D-lactic acid industrial production.

关 键 词：D-乳酸生产菌 分离 高光学纯度 发酵 木糖 

分 类 号：TS201.3[轻工技术与工程—食品科学]