大黄不同极性部位成分分析及抗氧化和抑菌活性研究  

Analysis of Bioactive Compounds,Antioxidant and Antibacterial Activity of Different Polar Parts of Rhubarb

作　　者：梁育桦 曹兴琴 毛明江 郭莹[1] LIANG Yuhua;CAO Xingqin;MAO Mingjiang(Zhejiang Chinese Medical University,Hangzhou(310053),China)

机构地区：[1]浙江中医药大学,杭州310053

出　　处：《浙江中医药大学学报》2024年第4期388-397,共10页Journal of Zhejiang Chinese Medical University

基　　金：浙江省中医药科学研究基金项目(2020ZB064);浙江中医药大学生命科学学院科研培育项目(2022BJ002);浙江省自然科学基金项目(LY16H280007)。

摘　　要：[目的]探究大黄不同极性部位有效成分含量与体外抗氧化和抑菌活性的相关性。[方法]采用90%乙醇回流提取大黄,采用氯仿、乙酸乙酯和正丁醇依次分级萃取,得到不同极性部位;以高效液相色谱(high performance liquid chromatography,HPLC)法测定其蒽醌类物质,以紫外分光度法测定总酚和总黄酮含量;以1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)法、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐[2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate),ABTS]法和总还原力法测定抗氧化活性,采用抑菌圈直径和生长曲线测定法探究大黄不同极性部位对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌的影响。[结果]正丁醇部位芦荟大黄素-8-O-葡萄糖苷和大黄酸-8-O-葡萄糖苷含量最多,峰面积分别为(2.72±0.07)mAU*min和(2.59±0.06)mAU*min,总酚和总黄酮含量最高,分别为(29.95±4.95)mg·g^(-1)和(35.71±3.26)mg·g^(-1);正丁醇部位的抗氧化活性和抑菌活性最强;芦荟大黄素-8-O-葡萄糖苷、大黄酸-8-O-葡萄糖苷、总酚和总黄酮含量与抗氧化活性正相关(P<0.05)。[结论]大黄总提物正丁醇部位的抗氧化和抑菌活性最强,可能与芦荟大黄素-8-O-葡萄糖苷、大黄酸-8-O-葡萄糖苷等成分有关,该结果对后续的深入研究具有指导意义。[Objective]To investigate the content of active ingredients in different polar parts of rhubarb and antioxidant and antibacterial activities in vitro.[Methods]Ninety percent ethanol reflux was used to extract rhubarb,and chloroform,ethyl acetate and n-butanol were used to extract different polar parts;anthraquinone compounds were determined by high performance liquid chromatography(HPLC);total phenol content(TPC)and total flavonoid content(TFC)were detected by UV spectrophotometry;1,1-diphenyl-2-picrylhydrazyl(DPPH)method,2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS)method,and total reducing power method were used to determine antioxidant activity.The effects of different polar parts of rhubarb on Escherichia coli,Staphylococcus aureus and Bacillus subtilis were measured by the diameter of inhibition zone and growth curve.[Results]The contents of aloe emodin-8-O-glucoside and rhein-8-O-glucoside were the highest in n-butanol part,with peak areas of(2.72±0.07)mAU*min and(2.59±0.06)mAU*min,respectively.TPC and TFC were the highest,with content of(29.95±4.95)mg·g^(-1)and(35.71±3.26)mg·g^(-1),respectively.The antioxidant and antibacterial activities of the n-butanol fraction were the strongest;antioxidant activity was positively correlated with aloe emodin-8-O-glucoside,rhein-8-O-glucoside,TPC and TFC(P<0.05).[Conclusion]The n-butanol fraction of rhubarb extract has the strongest antioxidant and antibacterial activity,which may be related to aloe emodin-8-O-glucoside and rhein-8-O-glucoside,and the results has guiding significance for further research.

关 键 词：大黄 HPLC 不同极性部位 抗氧化活性 总酚含量 总黄酮含量 抑菌活性 

分 类 号：R282[医药卫生—中药学]