氧化还原电位在微生物发酵中的应用  

Application of oxidation-reduction potential in microbial fermentation

作　　者：李子祥 奕栋 姜水琴 陈代杰[2] 谭俊 LI Zixiang;YI Dong;JIANG Shuiqin;CHEN Daijie;TAN Jun(China State Institute of Pharmaceutical Industry,Shanghai 201203,China;School of Pharmacy,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]中国医药工业研究总院,上海201203 [2]上海交通大学药学院,上海200240

出　　处：《中国酿造》2024年第5期25-31,共7页China Brewing

基　　金：微生物代谢国家重点实验室开放课题项目(MMLKF20-06);工业和信息化部“微生物发酵类原料药的发酵废水和菌渣处理技术项目”资助项目(CEIEC-2020-ZM02-0165);国家自然青年基金项目(22208217)。

摘　　要：氧化还原电位(ORP)作为一种可以实时监控发酵状况、高灵敏度的发酵参数,可以克服微氧、厌氧环境下溶氧电极应用的限制而应用于发酵。ORP波动既可以反映发酵液中氧化性或还原性的相对强弱,还与发酵过程中微生物生长状态密切相关。分析ORP数据可以判定微生物生长阶段,而调控ORP可以反向作用于微生物的基因表达和代谢活动,为发酵生产提供一种新颖的调控途径。该文系统介绍了微生物发酵中ORP的概念、波动原理及潜在的微观机制,总结了近年来ORP作为非调控参数时的发酵应用及新检测技术开发;同时针对ORP的调控方式、分析方法、产品开发以及菌种选育进行重点阐述,以期为ORP在微生物发酵中的深入研究提供理论基础。Oxidation-reduction potential(ORP),as a highly sensitive fermentation parameter that can monitor the fermentation status in real time,can be applied to fermentation by overcoming the limitations of the application of dissolved oxygen electrodes under microaerobic and anaerobic environments.ORP fluctuations is found to reflect the relative strength of oxidizing or reducing properties in the fermentation broth and are closely related to microbial growth status.Analysis of ORP data determines the growth phases of microorganisms,while regulation of ORP inversely influences gene expression and metabolic activities of microorganisms,providing a promising regulatory pathway for fermentation production.The concept,fluctuation principles,and potential microscopic mechanisms of ORP in microbial fermentation were introduced in this paper.Applications of ORP as a non-regulatory parameter in fermentation and advancements in new detection technologies were summarized.The regulation methods,analysis methods,product development,and strains breeding for ORP were mainly expounded,aiming to provide a theoretical foundation for further study of ORP in microbial fermentation.

关 键 词：氧化还原电位 发酵 调控 应用 

分 类 号：TS201.3[轻工技术与工程—食品科学] TQ920.1[轻工技术与工程—食品科学与工程]