飞龙掌血蜂蜜挥发性物质组成及关键香气物质分析  

Analysis of Volatile Composition and Key Aroma Compounds in Toddalia asiatica Lam.Honey

作　　者：姜澳 黎洪霞 刘肇龙 宋梅 李娜 陈兰珍[1,2] JIANG Ao;LI Hongxia;LIU Zhaolong;SONG Mei;LI Na;CHEN Lanzhen(State Key Laboratory of Resource Insects,Institute of Apicultural Research,Chinese Academy of Agricultural Sciences,Beijing 100093,China;Key Laboratory of Bee products for Quality and Safety Risk Assessment,Ministry of Agriculture and Rural Affairs,Beijing 100093,China)

机构地区：[1]资源昆虫高效养殖与利用全国重点实验室,中国农业科学院蜜蜂研究所,北京100093 [2]农业农村部蜂产品质量安全风险评估实验室(北京),北京100093

出　　处：《食品科学》2024年第11期175-185,共11页Food Science

基　　金：国家自然科学基金面上项目(32272402);国家蜂产业技术体系项目(CARS-44-KXJ8);国家蜂产品质量安全风险评估项目(GJFP20220305);中国农业科学院创新工程项目(CAAS-ASTIP-2023-IAR);中央级公益性科研院所基本科研业务费专项(MFSJKF2023)。

摘　　要：为明确中国特色中药材蜂蜜——飞龙掌血蜂蜜的挥发性物质组成及香气特征,利用阿贝折光仪、高效液相色谱仪、紫外分光光度计等分别对其水分含量、糖类含量、淀粉酶活性、抗氧化能力等指标进行测定。同时,利用顶空-固相微萃取结合气相色谱-四极杆飞行时间质谱仪对其挥发性成分进行定性定量分析。结果表明:飞龙掌血蜂蜜样品的水分质量分数为(18.71±1.49)%,果糖和葡萄糖质量分数分别为(35.92±2.70)%和(35.85±2.51)%,1,1-二苯基-2-三硝基苯肼自由基和2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)阳离子自由基的半清除浓度分别为(57.18±7.62)mg/mL和(247.68±40.50)mg/mL,淀粉酶活力为(11.84±3.38)mL/(g·h)。从飞龙掌血蜂蜜中共鉴定出176种挥发性物质,主要包括烃类52种、酯类29种、醇类21种、萜烯类21种、醛类17种和芳香族类12种等。其中苯甲醛、苯乙醇、1-辛烯-3-醇的含量较高,分别为8 181.9、4 483.1μg/kg和4 239.5μg/kg。经香气活度值分析鉴定出42种关键香气化合物(OAV>1),包括大马士酮、苯乙酸甲酯、α-水芹烯等,这些物质对飞龙掌血蜂蜜香气组成具有重要贡献。其中紫罗酮具有紫罗兰花香气,赋予了飞龙掌血蜂蜜独特的紫罗兰、覆盆子芳香味。飞龙掌血蜂蜜主要以花香味为主,辅以果香味、化学味和青香味的香气。综上,飞龙掌血蜂蜜的理化指标均满足相关标准,且挥发性成分种类丰富。本研究可为飞龙掌血蜂蜜品质评价提供数据支持,并为其他特色中药材蜂蜜的挖掘提供研究思路。In order to clarify the volatile composition and aroma characteristics of Toddalia asiatica Lam.honey,a Chinese specialty herbal honey,this study determined its selected physicochemical indexes such as water content,sugar content,amylase activity,and antioxidant capacity by using an Abbey refractometer,high-performance liquid chromatography(HpLC),and ultraviolet-visible spectrophotometry,respectively.Meanwhile,its volatile components were qualitatively and quantitatively analyzed using headspace-solid phase microextraction combined with gas chromatography-quadrupole time-of-flight mass spectrometry(HS-SpMe-GC-Q-tOF-MS).the results showed that T.asiatica Lam.honey contained(18.71±1.49)%moisture,(35.92±2.70)%fructose and(35.85±2.51)%glucose,and could scavenge 1,1-diphenyl-2-picrylhydrazyl(DppH)radical and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABtS)cation radical with half-maximum inhibitory concentration(IC50)values of(57.18±7.62)and(247.68±40.50)mg/mL,respectively,and its amylase activity was(11.84±3.38)mL/(g·h).A total of 176 volatile substances were identified from the honey,mainly including 52 hydrocarbons,29 esters,21 alcohols,21 terpenes,17 aldehydes,and 12 aromatics.Among them,the contents of benzaldehyde,phenylethanol and 1-octene-3-alcohol were high(8181.9,4483.1 and 4239.5μg/kg,respectively).totally 42 key aroma compounds,with odor activity values(OAV)greater than 1,were identified,including damastone,methyl phenylacetate andα-cresine,which contributed significantly to the aroma composition of T.asiatica Lam.honey.Among them,violonone had a violet flower-like aroma,being responsible for the unique violet-and raspberry-like aroma notes of T.asiatica Lam.honey.Overall,T.asiatica Lam.honey was dominated by floral aromas,along with fruity,chemical,and green aromas.In summary,the physicochemical indexes of the honey meet the relevant standards,and it contains a variety of volatile components.this study could provide data support for the quality evaluation of T.asiatica Lam.honey,as

关 键 词：飞龙掌血蜂蜜 顶空-固相微萃取-气相色谱-四极杆飞行时间质谱 挥发性成分 关键香气化合物 

分 类 号：S896.1[农业科学—特种经济动物饲养] O657.63[农业科学—畜牧兽医]