富含γ-氨基丁酸的萌发红小豆对T2DM小鼠肠道菌群的影响  

Effect of Rich GABA of Germinated Adzuki Bean on Intestinal Microflora in T2DM Mice

作　　者：姜秀杰[1,2] 张家瑜 李莹 迟晓星[1] 孙东波[3] 曹冬梅[1,4] 张东杰[1,4] JIANG Xiujie;ZHANG Jiayu;LI Ying;CHI Xiaoxing;SUN Dongbo;CAO Dongmei;ZHANG Dongjie(College of Food Science,Heilongjiang Bayi Agricultural University,Daqing 163319,China;Supervision,Inspection and Testing Center for Agricultural Products and Processed Products,Ministry of Agriculture and Rural Affairs,Daqing 163319,China;College of Animal Science and Technology,University of Heilongjiang Bayi Agricultural University,Daqing 163319,China;National Research for Grain Engineering Technology,Heilongjiang Bayi Agricultural University,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学食品学院,黑龙江大庆163319 [2]农业农村部农产品及加工品质量监督检验测试中心,黑龙江大庆163319 [3]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319 [4]黑龙江八一农垦大学国家杂粮工程技术研究中心,黑龙江大庆163319

出　　处：《食品工业科技》2024年第12期151-159,共9页Science and Technology of Food Industry

基　　金：黑龙江省博士后资助经费(LBH-Z23241);黑龙江省“百千万”工程科技重大专项(2021ZX12B06);黑龙江省自然科学基金项目(SS2022C001);学成、引进人才科研启动计划项目(XYB202210);黑龙江省杂粮生产与加工优势特色学科项目(2022-78);黑龙江八一农垦大学“三纵”基础培育项目(ZRCPY202122)。

摘　　要：为了研究富含GABA萌发红小豆对2型糖尿病(Diabetes Mellitus Type 2,T2DM)小鼠血糖水平及肠道菌群的影响,采用C57BL/6J小鼠为研究对象,通过高脂膳食+链脲菌素(Streptozocin,STZ)注射构建T2DM模型,选择不同剂量的富含GABA萌发红小豆对T2DM小鼠连续膳食干预6周,并利用16S rRNA测序技术对T2DM小鼠盲肠内容物的菌群结构和分布进行鉴定。结果显示:不同剂量富含GABA红小豆膳食干预可使T2DM小鼠FBG值明显下降,其中高剂量富含GABA红小豆(TF3)组FBG值为8.36±0.78 mmol/L,相比模型组(M)下降54.09%,干预效果最好。此外,TF3膳食可引起T2DM小鼠肠道菌群丰度发生显著(P<0.05)改变,门水平上Firmicutes丰度为35.96%,比M模型组下降53.17%,并可显著上调Bacteroidetes、Verrucomicrobia的菌群丰度(P<0.05)。表明TF3膳食改善糖脂代谢与Bacteroidetes、Verrucomicrobia优势菌丰度呈正相关,暗示高剂量富含GABA红小豆膳食可通过增加有益菌来缓解T2DM小鼠高血糖症状,为进一步解释T2DM与肠道菌群的关系提供参考。In order to study the effects of GABA-rich germinated adzuki bean on blood glucose level and intestinal flora in T2DM mice.C57BL/6J mice were used as subjects to construct T2DM model by high fat diet+STZ injection.Different doses of GABA-rich germinated adzuki bean were used to treat T2DM mice for 6 weeks and 16S rRNA sequencing technique was used to identify the microbial structure and distribution in the cecal contents of T2DM mice.The results showed that different doses of GABA-rich adzuki bean dietary intervention could significantly reduce the FBG value of T2DM mice,and the FBG value of high dose GABA-rich adzuki bean(TF3)group was 8.36±0.78 mmol/L,which was 54.09%,lower than that of model group(M).In addition,TF3 diet induced significant changes in intestinal flora abundance in T2DM mice.Firmicutes abundance at gate level was 35.96%,which decreased by 53.17%compared with M model group,and Bacteroidetes and Verrucomicrobia abundance were significantly up-regulated.In summary,the improvement of glucose and lipid metabolism by TF3 diet was significantly correlated with the abundance of dominant bacteria in Bacteroidetes and Verrucomicrobia,suggesting that high dose of GABA-rich adzuki bean diet could alleviate hyperglycemia symptoms in T2DM mice through beneficial bacteria,which provided reference for further explaining the relationship between T2DM and intestinal flora.

关 键 词：红小豆 萌发 Γ-氨基丁酸 2 型糖尿病 肠道菌群 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]